The Type III Secretion System plasmid pPHDPT3 of Photobacterium damselae subsp. piscicida is stable in Australian isolates due to conserved non-repetitive genomic architecture.

IF 2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2026-01-07 DOI:10.1093/femsle/fnag074

Oleksandra Rudenko, Laura Baseggio, Andrew C Barnes

引用次数: 0

Abstract

Photobacterium damselae subsp. piscicida (Pdp) is a host-adapted primary pathogen impacting finfish aquaculture worldwide, whose virulence evolution is driven by the mobilome. The pPHDPT3 plasmid encoding a type III secretion system is critical for Pdp virulence but unstable in vitro in European and Japanese isolates. Here we show that a stable ancestral variant is conserved in Australian isolates. The elusive pPHDPT3 variant has undergone gene loss and accumulated a ∼5.5 kb quadruple direct repeat, which could explain plasmid loss via the classic dimer catastrophe scenario. In addition, we hypothesize that a 189-aa serine recombinase encoded within this repeat, and also on pPHDP10 plasmid, may act as a plasmid resolvase, with its frequent loss further exacerbating the dimer catastrophe.

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少女叶光杆菌III型分泌系统质粒pPHDPT3的研究。由于保守的非重复基因组结构，piscicida在澳大利亚分离株中是稳定的。

光细菌damselae subsp。piscicida （Pdp）是一种影响世界范围内鱼类养殖的宿主适应性原致病菌，其毒力进化是由移动组驱动的。编码III型分泌系统的pPHDPT3质粒对Pdp毒力至关重要，但在欧洲和日本的体外分离株中不稳定。在这里，我们表明一个稳定的祖先变异是保守的澳大利亚分离。难以捉摸的pPHDPT3变体经历了基因丢失，并积累了约5.5 kb的四倍直接重复，这可以通过经典的二聚体灾难情景解释质粒丢失。此外，我们假设在这个重复序列中编码的189-aa丝氨酸重组酶，也在pPHDP10质粒上，可能作为质粒分解酶，其频繁丢失进一步加剧了二聚体灾难。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.