Bifidobacterium longum DPUB-15, screened by an ex vivo mesenteric lymph nodes cell model, alleviates allergic responses in casein-allergic Sprague-Dawley rats

IF 7.3 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness Pub Date : 2025-12-01 DOI:10.26599/fshw.2025.9250848

Zihao Guo, Feng Lu, Mingyang Ma, Yu Jiang, Rodríguez-Cervantes MA, Wenpu Yao, Guangqing Mu, Xuemei Zhu

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Abstract

Cow's milk allergy is a global health issue, and probiotic-based treatments may offer a promising therapeutic approach. Mesenteric lymph nodes (MLNs) play a crucial role in the development of food allergies. The present study aims to establish an ex vivo model based on rat-derived MLN cells to screen probiotic strains with potential to alleviate casein allergy and to investigate the effects in the casein-allergic rat model. The results showed that ex vivo screening via normal and casein-sensitized SD rat MLN cells identified three strains, with Bifidobacterium longum DPUB-15 selected as target strain demonstrating optimal probiotic properties. Subsequently, the present study demonstrated that both live and heat-killed Bifidobacterium longum DPUB-15 could alleviate casein allergy in rats. Notably, heat-killed Bifidobacterium longum DPUB-15 demonstrated superior effects, further downregulating IgG2a and histamine levels, improving ileal immune balance and colonic barrier function (ZO-1), and modulating intestinal microbiota by reducing the abundance of casein allergy-associated Lachnospirales_CAG-274 and increasing the abundance of Paraprevotella. In conclusion, this study provides an ex vivo model for screening anti-allergic probiotic strains and highlights Bifidobacterium longum DPUB-15 as a promising candidate for alleviating casein allergy, with heat-killed Bifidobacterium longum DPUB-15 showing potential for postbiotic development in allergy treatment.

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体外肠系膜淋巴结细胞模型筛选的长双歧杆菌DPUB-15可减轻酪蛋白过敏大鼠的过敏反应

牛奶过敏是一个全球性的健康问题，以益生菌为基础的治疗可能提供一种很有前途的治疗方法。肠系膜淋巴结（MLNs）在食物过敏的发展中起着至关重要的作用。本研究旨在建立基于大鼠源性MLN细胞的离体模型，筛选具有缓解酪蛋白过敏潜力的益生菌菌株，并研究其在酪蛋白过敏大鼠模型中的作用。结果表明，通过正常和酪蛋白致敏SD大鼠MLN细胞的离体筛选鉴定出3株菌株，其中长双歧杆菌DPUB-15为目标菌株，表现出最佳的益生菌特性。随后，本研究证明，活的和热杀的长双歧杆菌DPUB-15都能减轻大鼠的酪蛋白过敏。值得注意的是，热杀死的长双歧杆菌dpub15表现出了卓越的效果，它可以进一步下调IgG2a和组胺水平，改善回肠免疫平衡和结肠屏障功能（ZO-1），并通过降低酪蛋白过敏相关的Lachnospirales_CAG-274的丰度和增加Paraprevotella的丰度来调节肠道微生物群。总之，本研究为筛选抗过敏益生菌菌株提供了离体模型，并强调长双歧杆菌DPUB-15是缓解酪蛋白过敏的有希望的候选者，热杀长双歧杆菌DPUB-15在过敏治疗中显示出生物后发展的潜力。

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来源期刊

Food Science and Human Wellness Agricultural and Biological Sciences-Food Science

CiteScore

8.30

自引率

5.70%

发文量

审稿时长

28 days

期刊介绍： Food Science and Human Wellness is an international peer-reviewed journal that provides a forum for the dissemination of the latest scientific results in food science, nutriology, immunology and cross-field research. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. By their effort, it has been developed to promote the public awareness on diet, advocate healthy diet, reduce the harm caused by unreasonable dietary habit, and directs healthy food development for food industrial producers.