{"title":"One-pot bioprocessing: dynamics and opportunities for integrated multiproduct recovery-a review.","authors":"Daniel O Ojwang, Sammy K Chebon, Francis J Mulaa","doi":"10.1007/s00449-026-03341-7","DOIUrl":null,"url":null,"abstract":"<p><p>One-pot bioprocessing (OPB) represents an integrated strategy for biomass valorization within a single reactor rather than across sequential, isolated units. By consolidating formation-stage steps that are traditionally separated, OPB can lower capital intensity and reduce intermediate losses. It may also improve carbon utilization under specific conditions compared with conventional modular biorefineries optimized around a single product. Despite these advantages, OPB has yet to achieve robust scalability. This review examines the dynamic processes governing biomass fractionation into individual constituents and biocatalytic transformation in single-reactor systems. It synthesizes recent advances in metabolic engineering, process intensification, and dynamic flux control to assess how biological network behavior, thermodynamic feasibility, and reactor-scale transport phenomena jointly constrain feasible product combinations within integrated one-pot systems. Persistent limitations arising from metabolic trade-offs, physicochemical incompatibilities, and increasing control complexity are evaluated alongside emerging enabling strategies, including dynamic metabolic regulation, hybrid one-pot architectures, and digital bioprocess twins. This work provides a data-informed framework indicating that effective one-pot bioprocess design depends on aligning biological compatibility, control capacity, and operational robustness to support adaptive and anticipatory control of multiproduct formation dynamics.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprocess and Biosystems Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s00449-026-03341-7","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
One-pot bioprocessing (OPB) represents an integrated strategy for biomass valorization within a single reactor rather than across sequential, isolated units. By consolidating formation-stage steps that are traditionally separated, OPB can lower capital intensity and reduce intermediate losses. It may also improve carbon utilization under specific conditions compared with conventional modular biorefineries optimized around a single product. Despite these advantages, OPB has yet to achieve robust scalability. This review examines the dynamic processes governing biomass fractionation into individual constituents and biocatalytic transformation in single-reactor systems. It synthesizes recent advances in metabolic engineering, process intensification, and dynamic flux control to assess how biological network behavior, thermodynamic feasibility, and reactor-scale transport phenomena jointly constrain feasible product combinations within integrated one-pot systems. Persistent limitations arising from metabolic trade-offs, physicochemical incompatibilities, and increasing control complexity are evaluated alongside emerging enabling strategies, including dynamic metabolic regulation, hybrid one-pot architectures, and digital bioprocess twins. This work provides a data-informed framework indicating that effective one-pot bioprocess design depends on aligning biological compatibility, control capacity, and operational robustness to support adaptive and anticipatory control of multiproduct formation dynamics.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
