Mi Soon Park, Chaeyeon Lee, Junsoo Lee, Byung Hee Kim, Heon-Woong Kim, In-Hwan Kim
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引用次数: 0
Abstract
Docosahexaenoic acid (DHA)-enriched triacylglycerol (TAG) was successfully synthesized from DHA-enriched fatty acids with glycerol via enzyme-catalyzed esterification under vacuum conditions. Lipozyme 435 from Candida antarctica was employed as a biocatalyst. DHA-enriched fatty acids containing 85% DHA were prepared from tuna oil fatty acids through a combination of solvent crystallization and lipase-catalyzed ethanolysis. The optimal conditions were the temperature of 60°C, and the vacuum level of 2.67 kPa, respectively. The maximum conversion of approximately 85% was achieved at all enzyme loadings after 12 h, although the times required to reach equilibrium varied at each enzyme loading. Slight differences were observed in the fatty acid composition between the synthesized DHA-enriched TAG (82%) and the DHA-enriched fatty acids used as the substrate.
期刊介绍：
The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate.
JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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