Yao Chen, Dongyang Li, Yan Liu, Yongjia Cheng, Juan Zhang, Chaojie Li, Zhiwei Lei, Wen Yang
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引用次数: 0
Abstract
Background
Plants synthesize and emit a wide variety of volatile compounds that serve different defense-related functions in response to both abiotic and biotic stresses.
Results
In this study, 1-nonanol was identified as the most active volatile against Epicoccum sorghinum among those co-up-regulated in the tea cultivar of ‘Qianmei 601’ infected by three strains of E. sorghinum. Its antifungal results in vitro indicated that the minimum inhibitory concentration and the minimum bactericidal concentration were 0.1 mg/mL and 0.15 mg/mL, respectively. Scanning electron microscopy, transmission electron microscopy, calcofluor whiteand and propidium iodide staining solutions demonstrate that 1-nonanol inhibited the normal growth of E. sorghinum mycelia by affecting the cell membrane and cell wall structure of E. sorghinum mycelia. Additionally, exposure to 1-nonanol resulted in increased levels of intracellular malondialdehyde, soluble proteins, and chitinase, while superoxide dismutase content decreased. Transcriptome analysis revealed that regulatory genes associated with 1-nonanol were involved in starch and sucrose metabolism, glycerophospholipid metabolism, and fatty acid synthesis pathways. Treatment with 1-nonanol significantly up-regulated genes linked to exo-1,3-β-glucanase and hexokinase, while genes related to chitin synthase, aminophospholipid translocase, 3-oxoacyl-[acyl-carrier-protein] synthase, and mitochondrial 2-enoylthioester reductase were down-regulated.
Conclusions
These results indicate that 1-nonanol disrupts the integrity of cell walls and cell membranes, inhibiting the growth of normal hyphae and potentially causing cell death. This study is the first to confirm that 1-nonanol is a crucial component of the defense-related volatile compounds produced by ‘Qianmei 601’ in response to stress from E. sorghinum. We discussed these results and emphasized the importance of this interaction in plant–microbe interactions.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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