Comparative transcriptome analysis of kiwifruit reveals candidate genes involved in soft rot resistance against Botryosphaeria dothidea.

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2026-05-01 Epub Date: 2026-02-14 DOI:10.1002/jsfa.70519

Danni Lv, Yuxiao Li, Xiaoxia Zuo, Jing Yang, Guiqing Tu, Jing Wang, Zhipeng Cai, Yonggen Shen, Huaying Du, Wei Zhang, Liqin Zhu

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引用次数: 0

Abstract

Background: Soft rot caused by Botryosphaeria dothidea is a common disease in postharvest kiwifruit. Kiwifruit cultivars show varying susceptibility, but the molecular mechanisms underlying these differences are poorly understood.

Results: This study revealed that the 'Jinyan' (JY) kiwifruit cultivar exhibited greater resistance to B. dothidea and higher contents of total phenolics, flavonoids, and lignin compared to 'Jinkui' (JK). Transcriptome analysis revealed that 4011 genes were differentially expressed, with 1892 being upregulated and 2119 downregulated, between the resistant (JY) and susceptible (JK) kiwifruit cultivars on the first day post-inoculation. GO and KEGG enrichment analyses indicated that differentially expressed genes (DEGs) were primarily associated with defense response functions and metabolic pathways. The DEGs related to phenylalanine metabolism (DHQS, ADT, TAT, GOT2, 4CL, CHS, CHI, CCR, F5H, BGLU, BGLU2, BGLU4, POD12, POD55), calcium (Ca²⁺) signaling pathway (CALM2, CML23), transcription factors (WRKY22, MYC2), plant hormone signaling pathway (ATP7, ETR, EBF1_2, PP2C16, SAPK2), and MAPK signaling pathway (MKK4_5) were identified as key markers of resistance to B. dothidea. The expression patterns of these genes related to the key pathways were further validated by real-time quantitative polymerase chain reaction; most of these genes show higher expression levels in JY than in JK. In total, these candidate genes and their associated pathways were found to be associated with kiwifruit resistance to B. dothidea-induced soft rot.

Conclusion: This study revealed the functional genes and defense pathways involved in governing soft rot resistance in kiwifruit against B. dothidea. These results contribute to understanding the molecular differences in disease resistance between resistant and susceptible kiwifruit cultivars. © 2026 Society of Chemical Industry.

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猕猴桃抗软腐病候选基因的转录组比较分析。

背景：猕猴桃软腐病是猕猴桃采后常见病害。猕猴桃品种表现出不同的敏感性，但这些差异背后的分子机制尚不清楚。结果：金岩猕猴桃品种（JY）与金葵猕猴桃品种（JK）相比，具有更强的抗蚜能力和更高的总酚类物质、总黄酮和木质素含量。转录组分析显示，在接种后第一天，抗性（JY）和易感（JK）猕猴桃品种之间存在4011个基因的差异表达，其中1892个基因表达上调，2119个基因表达下调。GO和KEGG富集分析表明，差异表达基因（DEGs）主要与防御反应功能和代谢途径相关。鉴定出与苯丙氨酸代谢相关的DEGs （DHQS、ADT、TAT、GOT2、4CL、CHS、CHI、CCR、F5H、BGLU、BGLU2、BGLU4、POD12、POD55）、钙（Ca2+）信号通路（CALM2、CML23）、转录因子（WRKY22、MYC2）、植物激素信号通路（ATP7、ETR、ebf12、PP2C16、SAPK2）和MAPK信号通路（MKK4_5）为抗性的关键标记。通过实时定量聚合酶链反应进一步验证这些关键通路相关基因的表达模式；这些基因大部分在JY中的表达水平高于JK。结论：本研究揭示了猕猴桃抗软腐病的功能基因和防御途径。这些结果有助于了解抗感猕猴桃品种在抗病方面的分子差异。©2026化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.