Bhakti Nanaware, Pranav Shevkar, Venkata Gopal Ede, Ashutosh Goswami, Abhijeet S Kate
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引用次数: 0
Abstract
Introduction: Swertiamarin (SWE) is a bioactive secoiridoid glycoside derived from the medicinal plant Enicostemma littorale and is a promising candidate for anti-diabetic therapy. However, SWE have shown poor bioavailability and stability concerns.
Objective: This study aims to systematically investigate the stability of swertiamarin under controlled stress conditions according to International Council for Harmonisation (ICH) guidelines.
Materials and methods: A stability-indicating high-performance liquid chromatography (HPLC) method was developed and validated. Forced degradation of SWE was performed under heat (60°C), oxidative, and hydrolytic (acidic, basic, neutral) stress conditions.
Results: SWE underwent rapid degradation under hydrolytic stress, degrading completely within 20 minutes in base and within 2 hours in acid. These stress conditions produced four degradation products namely 5‑hydroxysecologanolic acid, loganic acid, sweritranslactone C, and sweritranslactone B. Overall, SWE was stable at 60°C and significantly degraded under other tested stress conditions such as acidic, basic, aqueous neutral and oxidative conditions.
Conclusion: The findings highlight the instability of SWE under stress environments and underscore the necessity of protective formulation strategies and controlled storage conditions to ensure its pharmaceutical efficacy in therapeutic applications.
期刊介绍：
Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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