Single-cell transcriptomics of chicken ovarian cancer identifying immune subsets with prognostic implications

IF 4.2 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2026-05-01 Epub Date: 2026-02-02 DOI:10.1016/j.psj.2026.106582

Guoqiang Zhu , Susanna Chau Yi Wang , Hongqin Yao , Jiliang He , Jiannan Zhang , Mao Zhang , Yajun Wang

{"title":"Single-cell transcriptomics of chicken ovarian cancer identifying immune subsets with prognostic implications","authors":"Guoqiang Zhu , Susanna Chau Yi Wang , Hongqin Yao , Jiliang He , Jiannan Zhang , Mao Zhang , Yajun Wang","doi":"10.1016/j.psj.2026.106582","DOIUrl":null,"url":null,"abstract":"<div><div>Ovarian cancer (OC) is a lethal gynecological malignancy with a low 5-year survival rate. The lack of preclinical models accurately capturing OC's early pathogenesis contributes to this poor prognosis. The domestic laying hen uniquely develops spontaneous OC, but its single-cell-level insights for pathogenesis have been lacking. Here, we present the first single-cell transcriptomic atlas of ovarian tissues from 110-week-old laying hens with or without OC. We annotated 12 core cellular lineages and characterized tumor-associated changes, including increased CD4+ and CD8+ <em>T</em> cells and reduced macrophages in OC samples. T cells sub-clustering revealed conserved cell subtype-specific pH homeostasis regulation profiles across species. Notably, gene signatures from the two dominant Cyto_CD8+ subsets exhibited opposing prognostic roles when validated against human OC datasets (GSE18520, GSE26712, GSE49997). This study confirms the laying hen as a valuable OC model by demonstrating cross-species conservation in cellular heterogeneity and oncogenic pathways, advancing it from a pathologically analogous system to a molecularly traceable tool.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"105 5","pages":"Article 106582"},"PeriodicalIF":4.2000,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0032579126002099","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/2/2 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Ovarian cancer (OC) is a lethal gynecological malignancy with a low 5-year survival rate. The lack of preclinical models accurately capturing OC's early pathogenesis contributes to this poor prognosis. The domestic laying hen uniquely develops spontaneous OC, but its single-cell-level insights for pathogenesis have been lacking. Here, we present the first single-cell transcriptomic atlas of ovarian tissues from 110-week-old laying hens with or without OC. We annotated 12 core cellular lineages and characterized tumor-associated changes, including increased CD4+ and CD8+ T cells and reduced macrophages in OC samples. T cells sub-clustering revealed conserved cell subtype-specific pH homeostasis regulation profiles across species. Notably, gene signatures from the two dominant Cyto_CD8+ subsets exhibited opposing prognostic roles when validated against human OC datasets (GSE18520, GSE26712, GSE49997). This study confirms the laying hen as a valuable OC model by demonstrating cross-species conservation in cellular heterogeneity and oncogenic pathways, advancing it from a pathologically analogous system to a molecularly traceable tool.

查看原文本刊更多论文

鸡卵巢癌单细胞转录组学鉴定与预后相关的免疫亚群

卵巢癌（OC）是一种致命的妇科恶性肿瘤，5年生存率低。缺乏准确捕捉卵巢癌早期发病机制的临床前模型导致了这种不良预后。国内产蛋母鸡是自发性OC的独特发病机制，但其单细胞水平的发病机制尚缺乏深入了解。在这里，我们展示了110周龄蛋鸡卵巢组织单细胞转录组图谱。我们注释了12个核心细胞系，并表征了肿瘤相关的变化，包括OC样本中CD4+和CD8+ T细胞增加和巨噬细胞减少。T细胞亚聚类揭示了物种间保守的细胞亚型特异性pH稳态调节谱。值得注意的是，当在人类OC数据集（GSE18520, GSE26712, GSE49997）中验证时，来自两个主要的Cyto_CD8+亚群的基因特征显示出相反的预后作用。本研究通过证明细胞异质性和致癌途径的跨物种保护，证实了蛋鸡是一个有价值的OC模型，将其从病理学上类似的系统推进到分子可追溯的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.