Technical note: contour plot visualization of the light adapted electroretinogram using a generalized additive model.

IF 2 4区医学 Q2 OPHTHALMOLOGY

Documenta Ophthalmologica Pub Date : 2026-02-01 Epub Date: 2026-01-18 DOI:10.1007/s10633-025-10078-3

Marek Brabec, Lynne Loh, Irene O Lee, Fernando Marmolejo-Ramos, David H Skuse, Dorothy A Thompson, Paul A Constable

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引用次数: 0

Abstract

Purpose: To describe a new method to visualize the amplitude profile of light adapted ERGs as a series of contour plots in response to a Flash Strength series.

Methods: Light adapted ERGs from a dataset of n = 88 Autism Spectrum Disorder (ASD) and n = 70 typically developing Control participants were used incorporating 10 flash strengths ranging from 12 to 446 Troland seconds (Td.s). The region chosen for analysis included the baseline, a-wave and ascending limb of the b-wave and included n = 1736 control and n = 1300 ASD waveforms. A Generalized Additive Model (GAM) with complexity penalized tensor product splines was applied to the waveform intervals.

Results: Contour plots derived from the ASD and Control groups revealed a pattern of reduced amplitude in the ascending limb of the b-wave for the ASD group. Representative responses were derived from the GAM model for discrete Flash Strengths to exemplify the changing profile of the ERG waveform. A contour plot of the derived z-scores provided a qualitative evaluation of the differences in the mean amplitudes which was concentrated on the b-wave.

Conclusions: The production of contour plots based on amplitude, time and Flash Strength with minimal a priori assumptions provide an additional approach to analyzing stimulus response data series and may support clinical applications. Additionally, the GAM-based methodology provides a tool for simultaneous investigation of the amplitude changes over time and Flash Strength which can be useful for theoretical purposes.

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技术说明：使用广义加性模型的光适应视网膜电图的轮廓图可视化。

目的：描述一种新的方法，将适应ERGs的光的振幅曲线可视化为响应闪光强度系列的一系列等高线图。方法：使用来自n = 88名自闭症谱系障碍（ASD）和n = 70名正常发展的对照组参与者的光适应eeg数据集，包括10种闪光强度，范围从12到446特兰秒（Td.s）。选取的分析区域包括基线波、a波和b波上升翼，包括n = 1736个对照波形和n = 1300个ASD波形。将复杂度惩罚张量积样条的广义加性模型（GAM）应用于波形区间。结果：来自ASD组和对照组的等高线图显示ASD组的b波上升肢振幅降低。代表性的反应是从离散闪光强度的GAM模型中得出的，以举例说明ERG波形的变化剖面。导出的z分数的等高线图提供了集中在b波上的平均振幅差异的定性评价。结论：基于振幅、时间和闪光强度的等高线图的生成，在最小的先验假设下，为分析刺激反应数据系列提供了一种额外的方法，并可能支持临床应用。此外，基于gam的方法提供了一种工具，可以同时研究振幅随时间的变化和闪光强度，这对理论目的很有用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Documenta Ophthalmologica 医学-眼科学

CiteScore

3.50

自引率

21.40%

发文量

审稿时长

>12 weeks

期刊介绍： Documenta Ophthalmologica is an official publication of the International Society for Clinical Electrophysiology of Vision. The purpose of the journal is to promote the understanding and application of clinical electrophysiology of vision. Documenta Ophthalmologica will publish reviews, research articles, technical notes, brief reports and case studies which inform the readers about basic and clinical sciences related to visual electrodiagnosis and means to improve diagnosis and clinical management of patients using visual electrophysiology. Studies may involve animals or humans. In either case appropriate care must be taken to follow the Declaration of Helsinki for human subject or appropriate humane standards of animal care (e.g., the ARVO standards on Animal Care and Use).