Cell wall patterning regulates plant stem cell dynamics

IF 47.3 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-12-04 DOI:10.1126/science.ady4102

Xianmiao Zhu, Xing Chen, Yangxuan Liu, Yimin Zhu, Geshuang Gao, Miao Lan, Yihao Fu, Yimin Gu, Han Han, Wenjuan Cai, Raymond Wightman, Mingjun Gao, Yiliang Ding, Weibing Yang

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引用次数: 0

Abstract

The plant cell wall regulates development through spatiotemporal modulation of its chemical and mechanical properties. Pectin methylesterification is recognized as a rheological switch controlling wall stiffness. Here, we reveal a bimodal methylesterification pattern in the shoot meristem: Mature walls exhibit high methylesterification, whereas demethylesterified pectins are deposited at new cross walls. This spatial heterogeneity is established through nuclear sequestration of PECTIN METHYLESTERASE5 (PME5) mRNA. MYB3R4-driven transcription, combined with RZ-1B/1C-mediated retention, creates a mitotically associated PME5 mRNA reservoir in the nucleus. Nuclear envelope disassembly synchronizes PME5 messenger RNA (mRNA) release with cell plate formation, enabling precise demethylesterification at division planes. Perturbation of this spatial control compromises stem cell maintenance or breaks division patterning. Our study uncovers an mRNA compartmentalization mechanism that couples stem cell dynamics with pectin modification.

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细胞壁模式调节植物干细胞动力学

植物细胞壁通过其化学和机械特性的时空调节来调节发育。果胶甲基化反应被认为是控制壁刚度的流变开关。在这里，我们揭示了芽分生组织中的双峰甲基化模式：成熟的壁表现出高的甲基化，而去甲基化的果胶沉积在新的交叉壁上。这种空间异质性是通过PME5 mRNA的核固存建立的。myb3r4驱动的转录，结合RZ-1B/ 1c介导的保留，在细胞核中创建了一个有丝分裂相关的PME5 mRNA库。核膜分解使PME5信使RNA （mRNA）的释放与细胞板形成同步，从而在分裂平面上实现精确的去甲基化。这种空间控制的扰动危及干细胞的维持或破坏分裂模式。我们的研究揭示了干细胞动力学与果胶修饰耦合的mRNA区区化机制。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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