Advances in acetylcholinesterase-based biosensing technologies for organophosphorus pesticide detection: A comprehensive review (2020–2024)

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-10-19 DOI:10.1016/j.foodchem.2025.146769

Qian Zhang , Jing Yu , Yanping Wei, Rong Yang, Jin Liu, Yangyang Su, Die Gao, Jing Zeng

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Abstract

This review comprehensively summarizes recent advances (2020–2024) in acetylcholinesterase-based biosensing technologies for detecting organophosphorus pesticides in food and environmental matrices. The critical role of innovative functional materials in enhancing biosensor performance through improved enzyme immobilization, signal amplification, and anti-interference capabilities is highlighted. The principles of acetylcholinesterase inhibition, simplified sample pretreatment, and various immobilization strategies are discussed. Electrochemical, optical, dual-mode, and capillary electrophoresis–based biosensing modalities are compared, emphasizing their respective advantages and limitations. Despite their high sensitivity, portability, and cost-effectiveness, acetylcholinesterase-based biosensing technologies face challenges in terms of specificity, anti-interference capabilities, and reproducibility. Strategies to overcome these limitations include developing engineered enzymes, integrating microfluidic pretreatment, employing eco-friendly nanomaterials, and implementing multisignal calibration and intelligent sensing systems. These advancements support a collaborative “screening–confirmation” framework that combines rapid biosensing with confirmatory liquid chromatography/gas chromatography–mass spectrometry techniques, significantly enhancing capabilities in food safety monitoring and environmental protection.

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基于乙酰胆碱酯酶的有机磷农药生物传感技术研究进展综述（2020-2024）

本文综述了基于乙酰胆碱酯酶的生物传感技术在食品和环境基质中有机磷农药检测方面的最新进展（2020-2024）。强调了创新功能材料通过改进酶固定、信号放大和抗干扰能力来增强生物传感器性能的关键作用。讨论了乙酰胆碱酯酶抑制原理、简化样品预处理和各种固定策略。比较了电化学、光学、双模和基于毛细管电泳的生物传感方式，强调了它们各自的优势和局限性。尽管具有高灵敏度、便携性和成本效益，但基于乙酰胆碱酯酶的生物传感技术在特异性、抗干扰能力和可重复性方面面临挑战。克服这些限制的策略包括开发工程酶，集成微流体预处理，采用环保纳米材料，实施多信号校准和智能传感系统。这些进步支持了一个协作的“筛选-确认”框架，该框架将快速生物传感与确认性液相色谱/气相色谱-质谱技术相结合，显著提高了食品安全监测和环境保护的能力。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.