Flexible nanoelectronics reveal arrhythmogenesis in transplanted human cardiomyocytes.

IF 45.8 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-10-16 DOI:10.1126/science.adw4612

Junya Aoyama,Ren Liu,Xinhe Zhang,Anthony Y Zhu,Pichayathida Luanpaisanon,Nivedhitha Velayutham,Jessica C Garbern,Fang Cao,Irving Barrera,Hannah Fandl,Morgan Sokol,Satvik Dasariraju,Eun Seok Gil,Elton Aleksi,Toshi Amanuma,Jeffrey J Saucerman,Fei Chen,Jia Liu,Richard T Lee

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Abstract

The transplantation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offers a potential treatment for heart failure, but arrhythmogenic automaticity arising from transplanted cells can arise. In this study, we investigated the effects of RADA16, a clinically approved self-assembling peptide that forms nanofibers after injection, on the vascularization, myofibril structure, and electrophysiological adaptation of hiPSC-CMs transplanted into rat hearts. RADA16 accelerated the transition of hiPSC-CMs toward adult-like gene expression profiles, enhanced sarcomere organization, and improved vascularization in the transplanted site. Flexible mesh nanoelectronics revealed fibrillation of transplanted hiPSC-CMs within the beating recipient heart, and RADA16 dramatically reduced the automaticity of hiPSC-CMs. Our findings demonstrate the potential of self-assembling nanofibers to advance cardiac cell therapy and how flexible mesh nanoelectronics technology could improve safety.

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柔性纳米电子学揭示移植的人心肌细胞的心律失常发生。

人类诱导多能干细胞来源的心肌细胞（hiPSC-CMs）的移植为心力衰竭提供了一种潜在的治疗方法，但移植细胞可能引起心律失常自动性。在这项研究中，我们研究了RADA16对hiPSC-CMs移植大鼠心脏血管化、肌原纤维结构和电生理适应的影响。RADA16是一种临床批准的自组装肽，注射后形成纳米纤维。RADA16加速了hiPSC-CMs向成人样基因表达谱的转变，增强了肌节组织，改善了移植部位的血管化。柔性网格纳米电子学揭示了移植的hiPSC-CMs在跳动的受体心脏内的颤动，RADA16显着降低了hiPSC-CMs的自动性。我们的发现证明了自组装纳米纤维在推进心脏细胞治疗方面的潜力，以及柔性网状纳米电子技术如何提高安全性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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