Discrimination of Russula vinosa Lindblad from different origins by lipidomics analysis using UHPLC-HR-AM/MS/MS

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-09-29 DOI:10.1016/j.jfca.2025.108387

Dingkui Xiang , Yi Liu , Minjie Zhao , Yue Ma , Eric Marchioni , Heping Chen , Li Zhou

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引用次数: 0

Abstract

Although Russula vinosa Lindblad (RVL) is widely valued as a nutritious edible mushroom worldwide, its quality varies considerably depending on geographical origin. Due to the stronger reputation of certain producing regions, significant price differences exist for RVL from different sources, which has led to instances of economically motivated adulteration, where inferior products are misrepresented as premium ones. In this study, we employed ultra-high-performance liquid chromatography coupled with quadrupole-Exactive-Orbitrap tandem mass spectrometry (UHPLC-QE Orbitrap/MS/MS) to characterize the lipid molecular species of RVL from different origins. A total of 186 molecular species across 21 lipid classes were identified. Combined with chemometrics, 33 lipid molecule species including PE 16:0/18:2, PE 18:2/18:2, HexCer 19:0;2 O/18:2;O, Cer 21:0;3 O/20:0;(2OH) and PI 16:0/18:2 were selected as potential biomarkers for geographic differentiation. This study represents the first comprehensive lipidomic analysis of the lipid profiles of RVL from major producing regions in China. It highlights systematic variations in lipid composition across different geographical origins and provides a scientific basis for verifying the provenance of this edible fungus. These findings support the protection of geographical indications (GIs) and help combat fraudulent labeling practices.

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采用UHPLC-HR-AM/MS/MS技术对不同产地的冬菇进行脂质组学分析

虽然Russula vinosa Lindblad （RVL）作为一种营养丰富的食用菌在世界范围内被广泛重视，但其质量因地理产地而异。由于某些生产地区的声誉较好，不同来源的RVL存在显着的价格差异，这导致了出于经济动机的掺假事件，即劣质产品被歪曲为优质产品。本研究采用超高效液相色谱-四极杆精确-轨道rap串联质谱法（UHPLC-QE Orbitrap/MS/MS）对不同来源的RVL脂质分子种类进行了表征。共鉴定出21个脂类186个分子种。结合化学计量学，得到了PE 16:0/18:2， PE 18:2/18:2, HexCer 19:0, 2 O/18:2；0, Cer 21:0;3 0 /20:0；（2OH）和PI 16:0/18:2作为地理分化的潜在生物标志物。本研究首次对中国主要产区的RVL进行了全面的脂质组学分析。它突出了不同地理来源的脂质组成的系统变化，并为验证这种食用菌的来源提供了科学依据。这些发现为保护地理标志提供了支持，并有助于打击标签欺诈行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.