Rapid detection of the inclusion of cassava starch in food and medicine products using Proofman-ladder shape melting temperature isothermal amplification method

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-10-04 DOI:10.1016/j.jfca.2025.108410

Pengfei Cui , Zhenqing Hu , Jiayi Zhou , Qingyuan Zhou , Yaqi Zhao , Wei Yao , Shanlu Guo , Fugang Xiao , Bailing Yin , Deguo Wang

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引用次数: 0

Abstract

Cassava starches are mostly added in high-value food and medicine starches including sweet potato, lotus rhizome, and common yam rhizome to gain economic profits. To rapidly detect cassava starch adulteration in various food and medicine samples, this research establishes an analysis method relied on proofreading enzyme-mediated probe cleavage (Proofman) coupled with ladder-shape melting temperature isothermal amplification (LMTIA). The optimal detection temperature of this Proofman-LMTIA method was 62℃ and the reaction could be finished within 20 min with a detection sensitivity of 100 pg/μL genomic cassava DNAs. Nine different species were collected and verified for the specificity of cassava ITS primers and probes. The detection limit of cassava DNAs derived from artificially premixed starch powders was 1 % (w/w). To demonstrate the effectiveness of the Proofman-LMTIA assay, 33 commercial products from food and medicine sectors were gathered and examined for the authentication of cassava contents, among these, 16 samples contained cassava components. Overall, the method developed here is suitable for accurate and quick detection of cassava items in food and medicine samples. The popularization of this Proofman-LMTIA technique will boost the starch quality management, offer instructions to authenticate cassava components, and facilitate the supervision of the food and medicine market.

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用proofman -梯形熔融温度等温扩增法快速检测食品药品中木薯淀粉的夹杂物

木薯淀粉多被添加到甘薯、莲藕、山药等高价值食品和医药淀粉中，以获得经济效益。为了快速检测各种食品和药品样品中的木薯淀粉掺假，本研究建立了一种基于校对酶介导的探针裂解（Proofman）结合梯形熔化温度等温扩增（LMTIA）的分析方法。该方法的最佳检测温度为62℃，反应在20 min内完成，检测灵敏度为100 pg/μL。收集了9种不同的木薯ITS引物和探针，并对其特异性进行了验证。人工预混淀粉粉提取的木薯dna检出限为1 % （w/w）。为了证明Proofman-LMTIA检测方法的有效性，我们收集了来自食品和医药行业的33种商业产品，并对其进行了木薯含量认证，其中16种样品含有木薯成分。总体而言，本方法适用于食品和药品样品中木薯类物质的准确、快速检测。该Proofman-LMTIA技术的推广将促进淀粉质量管理，为木薯成分的鉴定提供指导，促进食品药品市场的监管。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.