The AcMADS45-AcMADS64 Module Modulates Carotenoid Accumulation by Directly Regulating Carotenogenic Genes in Kiwifruit.

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-12 DOI:10.1021/acs.jafc.5c08278

Xiaoli Zhang,Zhiyi Lin,Xinling Liu,Wenjie Jiang,Dan Xu,Xiaowei Yan,Fan Tang,Mingzhang Li,Yue Xie,Lijin Lin,Jin Wang,Dong Liang,Hui Xia

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引用次数: 0

Abstract

Carotenoids are essential nutrient components and flesh pigments in kiwifruit. However, the regulatory mechanisms underlying carotenoid biosynthesis in kiwifruit remain elusive. In this study, we identified a transcription factor, AcMADS45, that is specifically expressed in fruit and exhibits a strong positive correlation with carotenoid accumulation. Overexpression of AcMADS45 in kiwifruit resulted in increased carotenoid content and upregulation of carotenoid biosynthesis genes. In contrast, virus-induced gene silencing (VIGS) of AcMADS45 reduced carotenoid levels and downregulated the expression of carotenoid biosynthesis genes, particularly AcBCH1/2. Further experiments using Y1H, EMSA, and luciferase assays confirmed that AcMADS45 directly binds to the AcBCH1/2 promoters to activate their transcription. In addition, we demonstrated that AcMADS64 acts as a positive transcriptional activator and interacts with AcMADS45 to synergistically enhance AcBCH1 expression, thereby increasing the carotenoid content in kiwifruit calli. These findings provide new insights into the molecular regulation of carotenoid accumulation in nonmodel plants.

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AcMADS45-AcMADS64模块通过直接调控猕猴桃类胡萝卜素基因调控类胡萝卜素积累。

类胡萝卜素是猕猴桃中必需的营养成分和果肉色素。然而，猕猴桃类胡萝卜素生物合成的调控机制尚不清楚。在这项研究中，我们发现了一个转录因子AcMADS45，它在水果中特异性表达，并与类胡萝卜素的积累表现出强烈的正相关。AcMADS45在猕猴桃中的过表达导致类胡萝卜素含量增加，类胡萝卜素生物合成基因上调。相比之下，病毒诱导的AcMADS45基因沉默（VIGS）降低了类胡萝卜素水平，下调了类胡萝卜素生物合成基因的表达，尤其是AcBCH1/2。进一步的Y1H、EMSA和荧光素酶实验证实，AcMADS45直接结合到AcBCH1/2启动子上，激活它们的转录。此外，我们证实AcMADS64作为一个正转录激活因子，与AcMADS45相互作用，协同增强AcBCH1的表达，从而增加猕猴桃愈伤组织中类胡萝卜素的含量。这些发现为非模式植物类胡萝卜素积累的分子调控提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.