Tumor Microenvironment T-cell Heterogeneity and Prognostic Model Construction in Laryngeal Squamous Cell Carcinoma by Single-cell and Bulk RNA Sequencing.
IF 1.6 4区 医学Q3 COMPUTER SCIENCE, INTERDISCIPLINARY APPLICATIONS
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引用次数: 0
Abstract
Objective: Characterize T-cell profiles in laryngeal squamous cell carcinoma (LSCC) using single-cell RNA sequencing and develop a prognostic model.
Methods: ScRNA-seq of LSCC tissues identified T-cell subtypes and marker genes. A prognostic model was constructed using COX and LASSO regressions with TCGA data, validated through bootstrap resampling and GEO dataset.
Results: Seven T-cell subtypes were detected. The model (HNRNPM, HSPH1, JMJD6, KDM5C, LCK) effectively predicted prognosis and correlated with immune infiltration, tumor mutational burden, and drug sensitivity.
Conclusion: This study provides insights into T-cell heterogeneity in LSCC and establishes a validated prognostic risk model for predicting survival and therapeutic outcomes.
期刊介绍：
The primary aims of Computer Methods in Biomechanics and Biomedical Engineering are to provide a means of communicating the advances being made in the areas of biomechanics and biomedical engineering and to stimulate interest in the continually emerging computer based technologies which are being applied in these multidisciplinary subjects. Computer Methods in Biomechanics and Biomedical Engineering will also provide a focus for the importance of integrating the disciplines of engineering with medical technology and clinical expertise. Such integration will have a major impact on health care in the future.
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