Sulfated Polysaccharides From Gracilaria gracilis (Red Seaweed) Restores Testicular Glucose Metabolism by Improving FSH and Insulin Signaling in Rats With Type 2 Diabetes

IF 4.2 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research Pub Date : 2025-10-06 DOI:10.1002/mnfr.70280

Ochuko L. Erukainure, Tosin A. Olasehinde, Olanrewaju S. Olayeriju, Bukola Omotoso, Md. Shahidul Islam, Ademola O. Olaniran

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Abstract

Testicular glucose homeostasis plays important roles in testicular energy metabolism and male fertility, but it is altered in type 2 diabetes (T2D), leading to male infertility. In the present study, the therapeutic effect of sulfated polysaccharides (SPCs) from Gracilaria gracilis on testicular glucose metabolism was investigated in T2D rats. SPCs were administered to two groups of T2D rats at 150 and 300 mg/kg bodyweight, respectively. The T2D control group was administered water only, while metformin served as the control drug administered to the standard treatment group. Non‐T2D rats served as the normal group. After 5 weeks of treatment, the rats were sacrificed and their testes harvested and analyzed for insulin, FSH, glucose, and glutathione metabolisms. Treatment with SPCs led to a significant increase in insulin, IRS‐1, FSH, GLUT4, GSH, ATP levels, hexokinase, glucose 6‐phisphate dehydrogenase, glyoxalase, glutathione reductase, and glutathione peroxidase activities, while improving testicular morphology. SPCs further led to significant depletion in glycogen phosphorylase, glucose 6‐phosphatase, fructose‐1,6‐biphosphatase, aldose reductase, polyol dehydrogenase, ATPase, and ENTPDase activities. These results indicate the potentials of SPCs from G. gracilis to improve male fertility in T2D. This is evident by its ability to improve insulin‐FSH signaling, glucose, energy metabolisms, and testicular morphology.

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红藻硫酸酸化多糖通过改善2型糖尿病大鼠FSH和胰岛素信号恢复睾丸糖代谢

睾丸葡萄糖稳态在睾丸能量代谢和男性生育能力中起重要作用，但在2型糖尿病（T2D）中发生改变，导致男性不育。本实验研究了股薄草硫酸多糖（SPCs）对t2dm大鼠睾丸糖代谢的影响。两组T2D大鼠分别给予150和300 mg/kg体重的SPCs。T2D对照组仅给予水治疗，标准治疗组给予二甲双胍作为对照药物。非T2D大鼠为正常组。治疗5周后，处死大鼠，取其睾丸，分析胰岛素、促卵泡刺激素、葡萄糖和谷胱甘肽代谢。SPCs治疗导致胰岛素、IRS‐1、FSH、GLUT4、GSH、ATP水平、己糖激酶、葡萄糖6‐磷酸脱氢酶、乙草醛酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶活性显著增加，同时改善睾丸形态。SPCs进一步导致糖原磷酸化酶、葡萄糖6‐磷酸酶、果糖- 1,6‐双磷酸酶、醛糖还原酶、多元醇脱氢酶、atp酶和entpase活性显著降低。这些结果表明，股薄草SPCs具有提高T2D雄性生殖力的潜力。其改善胰岛素-卵泡刺激素信号、葡萄糖、能量代谢和睾丸形态的能力证明了这一点。

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来源期刊

Molecular Nutrition & Food Research 工程技术-食品科技

CiteScore

8.70

自引率

1.90%

发文量

250

审稿时长

1.7 months

期刊介绍： Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.