A fluorescence and colorimetric dual-mode immunoassay for sensitive detection of marbofloxacin based on gold and silver nanoclusters

IF 4.6 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-09-23 DOI:10.1016/j.jfca.2025.108366

Xiujin Chen, Biao An, Minghui Dai, Pingping Xiong, Yubing Bai, Huawei Niu, Zhaozhou Li, Yao Wang

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引用次数: 0

Abstract

Marbofloxacin (MBF) is widely used in veterinary medicine for the prevention and treatment of various infectious diseases. However, improper use of MBF may leave residues in edible animal tissues and milk, posing a potential hazard to human health. Here, we developed a fluorescence and colorimetric dual-mode immunoassay for the detection of MBF based on the inner filter effect between gold-silver bimetallic nanoclusters (AuAgNCs) and the oxidation products of 3,3′,5,5′-tetramethylbenzidine (oxTMB). In this immunoassay, horseradish peroxidase (HRP) catalyzes the oxidation of TMB to oxTMB, which can further quench the fluorescence of AuAgNCs. Thereby, the color change of oxTMB and the fluorescence change of AuAgNCs can be utilized as the dual signals for MBF analysis. Under optimal conditions, the detection limit of the fluorescence enzyme-linked immunosorbent assay was 9 ng L⁻¹, with a 50 % inhibitory concentration (IC50) of 0.80 µg L⁻¹. Additionally, this immunoassay demonstrated high specificity for MBF and satisfactory recovery rates in pork samples (fluorescence mode: 84.00–104.40 %; colorimetric mode: 90.54–107.63 %) and milk samples (fluorescence mode: 88.00–104.00 %; colorimetric mode: 86.15–115.22 %). Hence, the developed immunoassay has great application potential for sensitive detection of MBF residues in foods.

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基于金和银纳米团簇的荧光和比色双模免疫分析灵敏检测马布沙星

马布沙星（MBF）广泛应用于兽医学，用于预防和治疗各种传染病。然而，不当使用MBF可能会在食用动物组织和牛奶中留下残留物，对人体健康构成潜在危害。本文基于金银双金属纳米团簇（AuAgNCs）与氧化产物3,3 ',5,5 ' -四甲基联苯胺（oxTMB）之间的内部过滤效应，建立了一种荧光和比色双模式免疫检测MBF的方法。在该免疫分析中，辣根过氧化物酶（HRP）催化TMB氧化为oxTMB，从而进一步猝灭auagnc的荧光。因此，oxTMB的颜色变化和auagnc的荧光变化可以作为MBF分析的双重信号。在最佳条件下，荧光酶联免疫吸附法的检出限为9 ng L−1,50% %的抑制浓度（IC50）为0.80 µg L−1。此外，该免疫分析法对MBF具有高特异性，在猪肉样品（荧光模式：84.00-104.40 %；比色模式：90.54-107.63 %）和牛奶样品（荧光模式：88.00-104.00 %；比色模式：86.15-115.22 %）中具有令人满意的回收率。因此，该方法在食品中MBF残留的灵敏检测中具有很大的应用潜力。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.