Phage adsorption-driven colorimetric broad-spectrum detection of foodborne Enterobacter hormaechei using phage-modified NH2-MIL-88B(Fe) nanozyme

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-10-02 DOI:10.1016/j.foodchem.2025.146616

An-qi Tang, Xuechao Xu, Cao-wei Chen, Mengyuan Tan, Lu Gao, Zhen-quan Yang

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Abstract

Enterobacter hormaechei, A zoonotic pathogen with escalating prevalence in global food sources, severely jeopardizes food safety, necessitating the development of high-performance detection methods. Herein, a novel colorimetric detection strategy integrating phages and nanozymes was proposed for foodborne E. hormaechei. Initially, four homologous phages (EhpYZU05, EhpYZU08, EhpYZU9–1 and EhpYZU10), which were isolated using E. hormaechei YZU05 as the host bacterium, were modified on the surface of NH₂-MIL-88B(Fe) to fabricate four phage@NH₂-MIL-88B(Fe) nanozymes. These nanozymes exhibit peroxidase-like activity, catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) chromogenic reaction in presence of H₂O₂. With introduction of multiple host or non-host E. hormaechei strains, TMB chromogenic reaction catalyzed by phage@NH₂-MIL-88B(Fe) nanozymes can be specifically inhibited. This inhibition occurs due to the specific phage adsorption of phage@NH₂-MIL-88B(Fe) nanozymes towards these host or non-host E. hormaechei strains, indirectly blocking their catalytic sites. Leveraging this principle, colorimetric broad-spectrum detection of E. hormaechei was achieved. Additionally, this method demonstrated reliable recoveries (96.7 % ~ 103.3 %) when applied to detect real food matrices, highlighting its high reliability in practical scenarios. This approach not only clarifies the intrinsic mechanism of phage-modified nanozymes for target bacteria detection but also provides a robust and reliable method for early pathogen detection, thereby significantly contributing to food safety enhancement

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利用噬菌体修饰的NH2-MIL-88B（Fe）纳米酶，噬菌体吸附驱动比色法广谱检测食源性激素肠杆菌

贺氏肠杆菌是一种在全球食品来源中流行率不断上升的人畜共患病原体，严重危害食品安全，需要开发高性能的检测方法。本文提出了一种整合噬菌体和纳米酶的食源性催乳杆菌比色检测方法。首先，以贺氏E. hormaechei YZU05为宿主菌分离到的4个同源噬菌体EhpYZU05、EhpYZU08、EhpYZU9-1和EhpYZU10，在NH2-MIL-88B（Fe）表面进行修饰，制备4个phage@NH2-MIL-88B（Fe）纳米酶。这些纳米酶表现出类似过氧化物酶的活性，在H2O2存在下催化3,3 ',5,5 ' -四甲基联苯胺（TMB）显色反应。通过引入多种宿主或非宿主荷氏大肠杆菌菌株，可以特异性抑制phage@NH2-MIL-88B（Fe）纳米酶催化的TMB显色反应。这种抑制作用的发生是由于phage@NH2-MIL-88B（Fe）纳米酶对这些宿主或非宿主荷氏大肠杆菌菌株的特异性噬菌体吸附，间接阻断其催化位点。利用这一原理，实现了麻麻杆菌的比色广谱检测。此外，该方法在实际食品基质检测中具有可靠的回收率（96.7 % ~ 103.3 %），在实际应用中具有较高的可靠性。该方法不仅阐明了噬菌体修饰纳米酶检测靶菌的内在机制，而且为早期病原菌检测提供了一种稳健可靠的方法，对提高食品安全具有重要意义

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.