Rapid Screening of an Anti-interference Histamine Biosensor Based on Modulation-Driven Arrays on a Chip.

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI:10.1021/acs.jafc.5c08561

Wei-Cheng Lin,Chun-Ting Hsieh,Yun-Yu Hsieh,Wei-Lun Yen

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Abstract

This study proposes a modulated pixel array design integrated with a membrane, polyvinyl chloride (PVC), aimed at accurate determination of the histamine concentration. The system employs a high-sensitivity analog front-end coupled with a double offset cancellation comparator, achieving a wide concentration range of 0.05-100 μM, a sensitivity of 5.1 ADC units per μM, and a voltage gain of approximately 12.25 mV/μM. Upon exposure to histamine in the presence of diamine oxidase (DAO), the sensor exhibited a substantial signal change of 61.6%. A significant inhibition is observed when the interference level is substantially high, specifically at a histamine-to-putrescine/cadaverine/tyramine ratio of 1:20. The high sensitivity and specificity of the proposed pixel array make it an ideal candidate for on-site histamine detection in food and fish markets. Its portable design facilitates swift screening, enabling timely intervention to reduce histamine-related health hazards and improve the effectiveness of food safety monitoring.

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基于芯片调制驱动阵列的抗干扰组胺生物传感器的快速筛选。

本研究提出了一种与聚氯乙烯（PVC）膜集成的调制像素阵列设计，旨在准确测定组胺浓度。该系统采用高灵敏度模拟前端和双偏置抵消比较器，可实现0.05 ~ 100 μM的宽浓度范围，灵敏度为5.1 ADC单位/μM，电压增益约为12.25 mV/μM。在二胺氧化酶（DAO）存在的情况下暴露于组胺，传感器显示出61.6%的显著信号变化。当干扰水平相当高时，特别是当组胺与腐胺/尸胺/酪胺的比例为1:20时，观察到显著的抑制作用。所提出的像素阵列的高灵敏度和特异性使其成为食品和鱼类市场现场组胺检测的理想候选者。其便携设计便于快速筛查，及时干预，减少与组胺相关的健康危害，提高食品安全监测的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.