Standardized Synergistic Strategy for Insect Growth Regulators: Incorporation of RNA Pesticides via a Co-Delivery Nanoplatform Achieves High Control Efficacy with Low-Dosage Application.

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-01 DOI:10.1021/acs.jafc.5c08250

Yunhui Zhang,Qinhong Jiang,Xiaoqian Wu,Xin Qian,Shiheng An,Jie Shen,Jianyu Meng,Shuo Yan

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引用次数: 0

Abstract

Insect growth regulators (IGRs) face limitations of slow action and high dosage, while RNA pesticides are constrained by the scarcity of effective RNAi targets. Here, we propose a standardized strategy to develop multicomponent nanopesticides by combining IGRs with RNA pesticides via a co-delivery nanoplatform. The star polycation (SPc) nanocarriers and diflubenzuron (DFB) were assembled into nanoscale DFB/SPc complexes, enhancing toxicity against green peach aphids by disrupting chitin biosynthesis. RNA-seq analysis identified chitin synthase (CHS) as a synergistic RNAi target. The hpCHS/SPc complex effectively silenced CHS, inducing more than 50% aphid mortality. Co-loading dsCHS and DFB produced stable nanoparticles with improved leaf adhesion, plant uptake, aphid cuticle contact, and translocation, achieving up to 82.2% mortality at only LC20 of free DFB. This work establishes a generalizable, scalable approach for designing multicomponent nanopesticides with high efficacy and low dosage for sustainable pest control.

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昆虫生长调节剂的标准化协同策略：通过共递送纳米平台加入RNA农药实现低剂量高控制效果。

昆虫生长调节剂（IGRs）面临着作用缓慢、剂量大的限制，而RNA农药则受到有效RNAi靶点缺乏的限制。在这里，我们提出了一种标准化的策略，通过共递送纳米平台将IGRs与RNA农药结合起来，开发多组分纳米农药。将星形多阳离子（SPc）纳米载体与二氟苯脲（DFB）组装成纳米级DFB/SPc复合物，通过破坏几丁质生物合成增强对青桃蚜虫的毒性。RNA-seq分析发现几丁质合成酶（CHS）是一个协同的RNAi靶点。hpCHS/SPc复合物有效地沉默了CHS，诱导蚜虫死亡率超过50%。共载dsCHS和DFB产生了稳定的纳米颗粒，改善了叶片粘附、植物吸收、蚜虫角质层接触和易位，仅在LC20的游离DFB下，死亡率高达82.2%。本研究为设计高效、低剂量、可持续防治害虫的多组分纳米农药建立了一种可推广、可扩展的方法。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.