Functional Characterization of a Fatty Acyl Reductase Identified from the Female Pheromone Gland of Mythimna loreyi (Lepidoptera: Noctuidae).

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI:10.1021/acs.jafc.5c08131

Liuyang Wang,Pei Guo,Xiaofang Zhang,Jianqing Guo,Runjie Meng,Jun Ning,Tao Zhang

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Abstract

Sex pheromones, which play a critical role in mediating sexual communication of Lepidoptera, are biosynthesized by a variety of particular enzymes in specialized pheromone glands (PGs). Among these enzymes, fatty acyl-CoA reductases (FARs) catalyze the reduction of fatty acyl precursors to their corresponding alcohols. In this study, we identified 32 FARs from the genome of Mythimna loreyi, an important agricultural pest. Among all MlorFARs identified, only MlorFAR1 showed a PG expression pattern and exhibited a positive response to pheromone biosynthesis activating neuropeptide (PBAN) injection. Silencing MlorFAR1 reduced pheromone titers by 70.4-88.9% and significantly reduced mating rates at the 48-72 h postinjection time point. Heterologous expression in yeast confirmed that MlorFAR1 preferentially converted medium-chain fatty acid methyl esters to alcohols. These findings establish MlorFAR1 as a pivotal enzyme in the biosynthesis of sex pheromones in M. loreyi, providing a framework for the development of innovative pest management strategies.

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一种从金翅目：夜蛾科雌费洛蒙腺中鉴定的脂肪酸酰还原酶的功能特征。

性信息素是鳞翅目昆虫在其特殊的信息素腺（PGs）中由多种特定的酶合成的，在性交流中起着至关重要的作用。在这些酶中，脂肪酰基辅酶a还原酶（FARs）催化脂肪酰基前体还原成相应的醇。在本研究中，我们从一种重要的农业害虫Mythimna loreyi的基因组中鉴定出32个FARs。在所有鉴定的MlorFARs中，只有MlorFAR1表现出PG表达模式，并对信息素生物合成激活神经肽（PBAN）注射表现出阳性反应。在注射后48 ~ 72 h，沉默MlorFAR1可使信息素滴度降低70.4 ~ 88.9%，显著降低交配率。在酵母中的异源表达证实，MlorFAR1优先将中链脂肪酸甲酯转化为醇。这些发现证实了MlorFAR1是洛雷依性信息素生物合成的关键酶，为开发创新的害虫管理策略提供了框架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.