Resistance Evolution and Fitness of Fusarium asiaticum from Fields to Succinate Dehydrogenase Inhibitor Pydiflumetofen in China.

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-02 DOI:10.1021/acs.jafc.5c07082

Zhili Yuan,Huina Ma,Niu Ma,Qi Wu,Zelong Peng,Chunjuan Yan,Yiping Hou

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引用次数: 0

Abstract

Pydiflumetofen, a succinate dehydrogenase inhibitor (SDHI), has been widely used to control Fusarium head blight (FHB) caused by Fusarium asiaticum since 2020. Resistance monitoring across different provinces in China from 2020 to 2023 detected no pydiflumetofen-tolerant or resistant F. asiaticum isolates. In 2024, 58 out of 539 F. asiaticum isolates (10.8%) collected from fields in China were identified as pydiflumetofen-tolerant (Pyd-TR), with their sensitivity distribution displaying a continuous rightward shift as EC50 values increased from 0.0168 to 0.0225 μg/mL and MIC values increased from 0.500 to 5.00 μg/mL. Pyd-TR exhibited growth, reproduction, and pathogenicity comparable to those of sensitive isolates. Cross-resistance was observed between pydiflumetofen and fluopyram but not with non-SDHI. No mutations were detected in SdhB/C1/C2/D of Pyd-TR. However, the expression of SdhC1 in Pyd-TR was significantly higher than in sensitive isolates after pydiflumetofen treatment. This study provides the first evidence of the early resistance evolution of F. asiaticum to pydiflumetofen in fields, which is associated with SdhC1 overexpression.

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中国田间亚洲镰刀菌对琥珀酸脱氢酶抑制剂吡氟醚的抗性进化及适应性研究

吡氟醚是琥珀酸脱氢酶抑制剂（SDHI），自2020年以来被广泛用于防治亚洲镰刀菌（Fusarium asiatium）引起的镰刀菌头疫病（FHB）。从2020年到2023年，在中国不同省份的耐药性监测中，未发现对嘧氟虫胺耐药或耐药的亚洲氟虫胺分离株。2024年，从中国田间采集到的539株亚洲氟虫蜱（F. asiatium）分离株中，鉴定出耐受性Pyd-TR （Pyd-TR）的有58株（10.8%），其敏感性分布呈连续的右移，EC50值从0.0168增加到0.0225 μg/mL， MIC值从0.500增加到5.00 μg/mL。Pyd-TR表现出与敏感分离株相当的生长、繁殖和致病性。在吡氟醚和氟吡兰之间观察到交叉抗性，但与非sdhi之间没有交叉抗性。Pyd-TR的SdhB/C1/C2/D未检测到突变。但经吡氟醚处理后，Pyd-TR中SdhC1的表达明显高于敏感株。本研究首次提供了田间亚洲灰螨对吡虫啉早期抗性进化的证据，这与SdhC1过表达有关。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.