On-Site Visualization and Sensing Platform for Detecting Genetically Modified Papaya "55-1" in Papaya-Derived Products.

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-01 DOI:10.1021/acs.jafc.5c07325

Lili Zhu,Fengyue Wei,Caiyao Wu,Can Wang,Gongwen He,Guiqin Yang,Zhiwen Pan,Juan Yao,Dagang Jiang

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引用次数: 0

Abstract

Genetically modified (GM) papaya "55-1" is the world's first papaya cultivar resistant to Papaya ringspot virus (PRSV). To facilitate government regulation and protect consumers' rights to informed choices, scientists are expected to develop a rapid and straightforward method for detecting "55-1". In this study, we established an on-site visualization and sensing platform based on recombinase polymerase amplification (RPA) to detect "55-1" in papaya-derived products. Amplified signals were generated exclusively in samples containing "55-1", with a detection limit of 20 copies, demonstrating high specificity and sensitivity. The entire process was completed within 40-60 min, indicating high efficiency. The platform was successfully applied to detect "55-1" in papaya leaves and derived products, producing results consistent with those obtained via polymerase chain reaction (PCR) analysis. To the best of our knowledge, this is the first platform enabling on-site visual detection of "55-1", and it provides a technical reference for the rapid detection of other GM plants.

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木瓜衍生产品中转基因木瓜“55-1”的现场可视化与传感平台

转基因木瓜“55-1”是世界上第一个抗番木瓜环斑病毒（PRSV）的番木瓜品种。为了促进政府监管和保护消费者知情选择的权利，科学家们有望开发一种快速而直接的方法来检测“55-1”。在这项研究中，我们建立了一个基于重组酶聚合酶扩增（RPA）的现场可视化和传感平台来检测木瓜衍生产品中的“55-1”。扩增信号只在含有“55-1”的样品中产生，检测限为20拷贝，具有较高的特异性和敏感性。整个过程在40-60分钟内完成，效率高。该平台成功应用于木瓜叶片及其衍生产品中“55-1”的检测，结果与聚合酶链反应（PCR）分析结果一致。据我们所知，这是第一个能够现场可视化检测“55-1”的平台，为其他转基因植物的快速检测提供了技术参考。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.