Licochalcone D Alleviates Radiation-Induced Intestinal Injury via SIRT3-Dependent Mitophagy: A Natural Phytochemical Strategy for Radioprotection.

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-10-01 DOI:10.1021/acs.jafc.5c05399

Jiaxin Zhang,Shengjie Ma,Zhen Wang,Pai Wang,Heshi Liu,Quan Wang

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Abstract

Oxidative stress, a key mechanism in radiation-induced intestinal injury (RIII), is triggered by ionizing radiation. Licochalcone D (LCD), a bioactive compound derived from licorice, exhibits potent antioxidant properties, yet its role and mechanism in RIII remain unclear. In this study, in vitro experiments revealed that the pretreatment of intestinal epithelial cells with LCD significantly enhanced cell viability, reduced radiation-induced DNA breaks, and suppressed reactive oxygen species (ROS) accumulation. In vivo, LCD administration mitigated radiation-induced body weight loss, preserved intestinal crypt integrity, and attenuated villous damage in irradiated mice. Mechanistically, LCD stabilizes SIRT3 protein to activate mitophagy, thereby eliminating radiation-generated ROS and subsequently alleviating DNA damage and preventing cell apoptosis. These findings broaden the potential applications of LCD in protecting against RIII and establish the SIRT3-mitophagy axis as a novel pharmacological target for preventing radiation-induced intestinal damage.

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甘草查尔酮D通过sirt3依赖的线粒体自噬减轻辐射诱导的肠道损伤：一种天然的辐射防护植物化学策略。

氧化应激是辐射诱发肠道损伤（RIII）的重要机制，电离辐射可诱发氧化应激。甘草查尔酮D （Licochalcone D， LCD）是一种从甘草中提取的生物活性化合物，具有较强的抗氧化性能，但其在iii期中的作用和机制尚不清楚。在本研究中，体外实验表明，用LCD预处理肠上皮细胞可显著提高细胞活力，减少辐射诱导的DNA断裂，抑制活性氧（ROS）积累。在体内，LCD可减轻辐射小鼠的体重减轻，保持肠隐窝完整性，并减轻绒毛损伤。在机制上，LCD稳定SIRT3蛋白激活线粒体自噬，从而消除辐射产生的ROS，从而减轻DNA损伤，防止细胞凋亡。这些发现拓宽了LCD在预防RIII方面的潜在应用，并建立了sirt3 -线粒体自噬轴作为预防辐射诱导肠道损伤的新药理学靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.