The effect of infant formula-based powder containing milk fat globule membrane on the 4-kingdom cornerstone microbiota in the feces and the immune ability of suckling rats.

IF 4.4 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Zihan Xia, Yushi Jin, Han Sun, Congcong Guo, Haiyan Xu, Longfei Zhang, Chenchen Zhang, Chengran Guan, Hengxian Qu, Yujun Huang, Wenlong Ma, Jilong Feng, Ruixia Gu, Dawei Chen
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Abstract

Growing evidence demonstrates that the 4-kingdom intestinal microbiota (FKIM) play a critical role in improving the body's immune processes. Three batches of experiments were conducted to study the effects of infant formula-based powder (IFBP) containing milk fat globule membrane (MFGM), human milk oligosaccharides, and probiotics, respectively, on the FKIM of suckling rats, with the IFBP serving as the control. Compared with the control, 3 functional components could increase the body weight and improve the composition of FKIM. Notably, the abundance and number of cornerstone species across the FKIM in the feces of suckling rats supplemented with MFGM were significantly higher than those in the other groups, and the levels of immune factors in the serum were significantly increased. After MFGM intervention, the abundance of cornerstone species of 99 genera in the feces was significantly correlated with the levels of immune factors in the serum. Among the cornerstone species, there are 11 bacteria, 6 eukaryotes, 2 archaea, and 1 virus that serve as biomarkers to distinguish the fecal microbiota of suckling rats fed with IFBP containing and not containing MFGM. Milk fat globule membrane can improve immunity by regulating functional pathways such as NOD-like receptor signaling and primary immunodeficiency. Milk fat globule membrane can stabilize the cornerstone species in the suckling rats' feces and enhance their immune ability by modulating the intestinal cornerstone species related to serum immunity, and their functional pathways, providing a theoretical basis for developing infant formula with immune-regulating functions.

含乳脂球膜婴儿配方粉对哺乳大鼠粪便4界基础菌群及免疫能力的影响
越来越多的证据表明,4王国肠道微生物群(FKIM)在改善人体免疫过程中起着关键作用。本实验以乳脂球膜(MFGM)、人乳低聚糖和益生菌为对照,分别研究了含有乳脂球膜(MFGM)、人乳低聚糖和益生菌的婴儿配方粉(IFBP)对哺乳大鼠FKIM的影响。与对照组相比,3种功能成分均能增加体重,改善FKIM的组成。值得注意的是,添加MFGM的哺乳大鼠粪便中FKIM的基石物种丰度和数量显著高于其他组,血清中免疫因子水平显著升高。经MFGM干预后,粪便中99属基础种丰度与血清中免疫因子水平显著相关。在基础物种中,有11种细菌、6种真核生物、2种古细菌和1种病毒可作为区分饲喂含和不含MFGM IFBP的哺乳大鼠粪便微生物群的生物标志物。乳脂球膜可通过调节nod样受体信号和原发性免疫缺陷等功能途径提高免疫功能。乳脂球膜可通过调节与血清免疫相关的肠道基石物质及其功能通路,稳定哺乳大鼠粪便中的基石物质,增强其免疫能力,为研制具有免疫调节功能的婴幼儿配方奶粉提供理论依据。
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来源期刊
Journal of Dairy Science
Journal of Dairy Science 农林科学-奶制品与动物科学
CiteScore
7.90
自引率
17.10%
发文量
784
审稿时长
4.2 months
期刊介绍: The official journal of the American Dairy Science Association®, Journal of Dairy Science® (JDS) is the leading peer-reviewed general dairy research journal in the world. JDS readers represent education, industry, and government agencies in more than 70 countries with interests in biochemistry, breeding, economics, engineering, environment, food science, genetics, microbiology, nutrition, pathology, physiology, processing, public health, quality assurance, and sanitation.
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