Spinal Cord Injury in Real Time: Intra-Operative Ultrasound for Acute Phase Examination in Non-Human Primates.

IF 2.8 3区医学 Q3 NEUROSCIENCES

Brain Sciences Pub Date : 2025-09-17 DOI:10.3390/brainsci15091005

Eleni Sinopoulou, Michelle W Chow, Numaira Obaid, Emily Chong, Yvette S Nout-Lomas, Rachele Wurr, Ryan Macon, J Russell Huie, Adam R Ferguson, Mark H Tuszynski, Michael S Beattie, Jacqueline C Bresnahan, Carolyn J Sparrey

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引用次数: 0

Abstract

Background: A spinal cord contusion injury is among the most clinically relevant models for studying pathophysiology and for developing potential therapeutic interventions for spinal cord injuries (SCI).

Methods: In this study, we implemented an intra-operative ultrasound (IOU) approach to precisely locate and examine the lesion site at 5 and 10 min post-injury after a cervical hemi-contusion injury in a non-human primate (NHP) model. We assessed acute lesion progression from 5 to 10 min and then compared that to the lesion extent as measured by MRI 3 weeks later.

Results: We observed a small increase in the rostrocaudal and mediolateral lesion area (mm²) from 5 to 10 min and a further 26% increase in the mediolateral lesion extent when comparing 5 and 10 min to 3 weeks post-injury.

Conclusions: By enabling high-resolution ultrasound visualization of the hemicontusion lesion in vivo, this approach can provide critical insights into the early progression of SCI. It can help with further refining this preclinical SCI model and provide significant predictive value for the animals' recovery post-injury.

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脊髓损伤的实时：术中超声在非人类灵长类动物急性期检查。

背景：脊髓挫伤是研究病理生理学和开发潜在的脊髓损伤治疗干预措施的最具临床意义的模型之一。方法：在本研究中，我们采用术中超声（IOU）方法在非人灵长类动物（NHP）颈部半挫伤模型损伤后5和10分钟精确定位和检查病变部位。我们评估了5至10分钟的急性病变进展，然后将其与3周后MRI测量的病变程度进行比较。结果：我们观察到，在损伤后5至10分钟内，背侧和中外侧病变面积（mm2）略有增加，在损伤后5和10分钟至3周内，中外侧病变范围进一步增加26%。结论：通过高分辨率超声在体内显示半塌陷病变，这种方法可以为脊髓损伤的早期进展提供重要的见解。这有助于进一步完善临床前脊髓损伤模型，对动物损伤后的恢复具有重要的预测价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain Sciences Neuroscience-General Neuroscience

CiteScore

4.80

自引率

9.10%

发文量

1472

审稿时长

18.71 days

期刊介绍： Brain Sciences (ISSN 2076-3425) is a peer-reviewed scientific journal that publishes original articles, critical reviews, research notes and short communications in the areas of cognitive neuroscience, developmental neuroscience, molecular and cellular neuroscience, neural engineering, neuroimaging, neurolinguistics, neuropathy, systems neuroscience, and theoretical and computational neuroscience. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.