High-Fat-Diet-Induced Kidney Injury in Rats: The Role of Tart Cherry Supplementation.

IF 6.6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2025-09-10 DOI:10.3390/antiox14091102

Ilenia Martinelli, Proshanta Roy, Vincenzo Bellitto, Maria Vittoria Micioni Di Bonaventura, Carlo Cifani, Seyed Khosrow Tayebati, Daniele Tomassoni

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引用次数: 0

Abstract

The kidney plays a crucial role in the complex inter-organ communication that occurs during obesity, leading to the development of oxidative stress, inflammation, and fibrosis. Dysfunction of the transient receptor potential (TRP) ion channels contributes to this pathophysiology. This study was designed to evaluate the effects of antioxidant-rich fruit tart cherry (Prunus cerasus L.) on kidney morphology and protein expression in rats with diet-induced obesity (DIO). Methods include histological staining and immunohistochemical and Western blot assays. Obese rodents were fed with seed powder (DS) and seed powder plus juice (DJS) of the tart cherry. Results demonstrated that rats fed a high-fat-diet (HFD) showed a significant reduction in renal expression of the pro-inflammatory cytokines interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) following tart cherry supplementation. Furthermore, the study provided evidence that TRP channels, specifically TRP canonical 1 (TRPC1) and TRP melastatin 2 (TRPM2), were significantly upregulated in obese animals (p < 0.05 vs. CHOW rats) and markedly downregulated following tart cherry supplementation (p < 0.05 vs. DIO rats). In conclusion, these TRP proteins offer new insights for identifying targets and biomarkers for developing therapeutic strategies against HFD-induced renal damage, characterized by glomerulosclerosis, fibrosis, and inflammation. Tart cherries supplementation exerted a protective effect on the kidneys by reducing protein oxidation and pro-inflammatory cytokine expression.

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高脂肪饮食引起的大鼠肾损伤：酸樱桃补充剂的作用。

肾脏在肥胖期间发生的复杂的器官间交流中起着至关重要的作用，导致氧化应激、炎症和纤维化的发展。瞬时受体电位（TRP）离子通道的功能障碍有助于这种病理生理。本研究旨在探讨富含抗氧化剂的酸樱桃（Prunus cerasus L.）对饮食性肥胖大鼠肾脏形态和蛋白质表达的影响。方法包括组织染色、免疫组化和Western blot检测。用酸樱桃籽粉（DS）和酸樱桃籽粉加果汁（DJS）分别饲喂肥胖大鼠。结果表明，饲喂高脂肪饮食（HFD）的大鼠在补充酸樱桃后，肾脏中促炎细胞因子白细胞介素-1β (IL-1β)和白细胞介素-6 （IL-6）的表达显著降低。此外，该研究还提供了证据，证明肥胖动物的TRP通道，特别是TRP canonical 1 （TRPC1）和TRP melastatin 2 （TRPM2）在补充酸樱桃后显著上调（p < 0.05），而在补充酸樱桃后显著下调（p < 0.05）。总之，这些TRP蛋白为确定目标和生物标志物提供了新的见解，以制定针对以肾小球硬化、纤维化和炎症为特征的hfd诱导的肾损害的治疗策略。酸樱桃补充剂通过减少蛋白质氧化和促炎细胞因子表达对肾脏产生保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.