{"title":"Potential of some lactic acid bacteria inoculants in the bioremediation of cyanogenic glycosides in sorghum straw silage","authors":"Yuxin Sun, Tingyu Liu, Kai Shi","doi":"10.1186/s40538-025-00823-w","DOIUrl":null,"url":null,"abstract":"<div><p>Sorghum is a vital economic crop in China, generating substantial amounts of crop residues annually, which necessitates research into its comprehensive utilization. Cyanogenic glycosides, a class of metabolites widely distributed in sorghum and other plant species, tend to release hydrogen cyanide under enzymatic action, posing a potential poisoning risk to animals. In the present study, to enhance the fermentation quality of sorghum straw silage, β-glucosidase-producing lactic acid bacteria isolated from fresh sorghum straw were employed as inoculants. A systematic investigation was conducted on the microbial community structure and metabolomic profiles following 60 days of fermentation, utilizing untargeted metabolomics analysis to elucidate the mechanisms by which β-glucosidase-producing lactic acid bacteria influence sorghum straw silage fermentation quality, as well as the degradation of dhurrin and reduction of hydrocyanic acid levels in sorghum. The results demonstrated that the silage fermentation quality in the lactic acid bacteria-supplemented experimental group was significantly improved compared to the control group. Attributed to the modulation of metabolic pathways, the levels of dhurrin and hydrocyanic acid exhibited a significant decrease with extended ensiling duration. At the metabolite level, biological pathways involved in steroid biosynthesis, terpenoid synthesis, and carotenoid synthesis were upregulated during different stages of ensiling. These metabolites not only enhance the nutritional value of the feed, but also possess anti-inflammatory and health-promoting properties, thereby further underscoring the positive regulatory role of lactic acid bacteria in the silage process. </p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-025-00823-w","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-025-00823-w","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
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Abstract
Sorghum is a vital economic crop in China, generating substantial amounts of crop residues annually, which necessitates research into its comprehensive utilization. Cyanogenic glycosides, a class of metabolites widely distributed in sorghum and other plant species, tend to release hydrogen cyanide under enzymatic action, posing a potential poisoning risk to animals. In the present study, to enhance the fermentation quality of sorghum straw silage, β-glucosidase-producing lactic acid bacteria isolated from fresh sorghum straw were employed as inoculants. A systematic investigation was conducted on the microbial community structure and metabolomic profiles following 60 days of fermentation, utilizing untargeted metabolomics analysis to elucidate the mechanisms by which β-glucosidase-producing lactic acid bacteria influence sorghum straw silage fermentation quality, as well as the degradation of dhurrin and reduction of hydrocyanic acid levels in sorghum. The results demonstrated that the silage fermentation quality in the lactic acid bacteria-supplemented experimental group was significantly improved compared to the control group. Attributed to the modulation of metabolic pathways, the levels of dhurrin and hydrocyanic acid exhibited a significant decrease with extended ensiling duration. At the metabolite level, biological pathways involved in steroid biosynthesis, terpenoid synthesis, and carotenoid synthesis were upregulated during different stages of ensiling. These metabolites not only enhance the nutritional value of the feed, but also possess anti-inflammatory and health-promoting properties, thereby further underscoring the positive regulatory role of lactic acid bacteria in the silage process.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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