Whole genome characterization of Listeria welshimeri isolates recovered from cattle abattoirs and retailers in Gauteng province in South Africa

IF 1.7 3区农林科学 Q4 FOOD SCIENCE & TECHNOLOGY

Journal of Consumer Protection and Food Safety Pub Date : 2025-02-24 DOI:10.1007/s00003-025-01546-8

James Gana, Rian Ewald Pierneef, Nomakorinte Gcebe, Rebone Moerane, Abiodun Adewale Adesiyun

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Abstract

The study used whole-genome sequencing (WGS) to characterize 12 isolates of L. welshimeri recovered from the beef production chain in Gauteng province, South Africa. Two sequence types (STs) were identified, with ST3294 accounting for 75% (9/12) and ST1084 for 16.7% (2/12) of the isolates. Antimicrobial resistance (AMR) plasmids detected included conjugative plasmid (MOBP2; T_virB11; virb4) in 58.3% and AMR plasmid (NFO33156) in 41.7% of the isolates. Two AMR genes, fosX (25%) and vga (G) (100%), along with 11 virulence factors, were present in all 12 isolates of L. welshimeri. The phylogenomic tree of L. welshimeri clustering based on the ST according to the source, type, and class of beef and beef products revealed three groups: ST3294 (n = 9), ST1084 (n = 2), and unassigned ST (n = 1). The findings of this study demonstrate the potential for transferring genetic materials from non-pathogenic L. welshimeri to pathogenic L. monocytogenes within the same ecological niche.

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从南非豪登省的牛屠宰场和零售商中回收的威氏李斯特菌分离株的全基因组特征

该研究利用全基因组测序（WGS）对从南非豪登省牛肉生产链中回收的12株韦氏乳杆菌进行了鉴定。鉴定出2种序列类型（STs），其中ST3294占75% (9/12)，ST1084占16.7%（2/12）。检测到的抗菌素耐药性质粒包括结合质粒（MOBP2、T_virB11、virb4）（58.3%）和抗菌素耐药性质粒（NFO33156）（41.7%）。12株威氏乳杆菌均存在2个AMR基因，即fosX（25%）和vga (G)(100%)，以及11个毒力因子。根据牛肉和牛肉制品的来源、类型和类别，基于ST聚类的威尔士乳杆菌系统基因组树显示出3个类群：ST3294 （n = 9）、ST1084 （n = 2）和未分配ST （n = 1）。本研究结果表明，在同一生态位内，非致病性威氏乳杆菌的遗传物质有可能转移到致病性单核增生乳杆菌中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Consumer Protection and Food Safety Veterinary-Food Animals

CiteScore

3.70

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： The JCF publishes peer-reviewed original Research Articles and Opinions that are of direct importance to Food and Feed Safety. This includes Food Packaging, Consumer Products as well as Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering. All peer-reviewed articles that are published should be devoted to improve Consumer Health Protection. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of Food and Feed Safety issues on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of Food and Feed Safety and Consumer Health Protection. Manuscripts – preferably written in English but also in German – are published as Research Articles, Reviews, Methods and Short Communications and should cover aspects including, but not limited to: · Factors influencing Food and Feed Safety · Factors influencing Consumer Health Protection · Factors influencing Consumer Behavior · Exposure science related to Risk Assessment and Risk Management · Regulatory aspects related to Food and Feed Safety, Food Packaging, Consumer Products, Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering · Analytical methods and method validation related to food control and food processing. The JCF also presents important News, as well as Announcements and Reports about administrative surveillance.