A Comprehensive Study on the Disinfection Performance of Four Representative Atmospheric Pressure Plasma Sources

IF 2.5 3区物理与天体物理 Q3 ENGINEERING, CHEMICAL

Plasma Chemistry and Plasma Processing Pub Date : 2025-07-21 DOI:10.1007/s11090-025-10582-z

Dongxue Feng, Guoqiang Liu, Zhishang Wang, Di Dou, Dongping Liu

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Abstract

In this study, air transient spark discharge (Air-TSD) plasma, helium atmospheric pressure plasma jet (He-APPJ), air surface dielectric barrier discharge (Air-SDBD) plasma, and nitrogen micro hollow cathode discharge (N₂-MHCD) plasma were used to inactivate Escherichia coli (E. coli). The results showed that Air-SDBD plasma achieved a 6-log reduction of E. coli in 30 s, whereas the other three sources failed to achieve complete inactivation even after the treatment of 120 s. The concentrations of aqueous H₂O₂, NO₂⁻ and NO₃⁻ produced by Air-SDBD are 2–3 times higher than those of Air-TSD and He-APPJ. The concentration of O₃ produced by Air-SDBD is more than 4 times that of N₂-MHCD. To further explore the relationship between RONS produced by the four sources and E. coli inactivation, we analyzed the effects of the four sources on 12 representative amino acids. Liquid chromatography-mass spectrometry (LC–MS) analysis showed that amino acids, including phenylalanine (Phe), methionine (Met), tryptophan (Trp), tyrosine (Tyr), glutamic (Glu), lysine (Lys), histidine (His), arginine (Arg), and cysteine (Cys), underwent significant oxidation after treatment of the four sources. And the reduction of Met, Trp and Cys after Air-SDBD treatment was more pronounced compared to the other three sources. Our results indicates that a strong correlation exists between the inactivation of E. coli and the modification of amino acids by RONS.

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四种典型常压等离子体源消毒性能的综合研究

本研究采用空气瞬态火花放电（air - tsd）等离子体、氦大气压等离子体射流（He-APPJ）、空气表面介质阻挡放电（air - sdbd）等离子体和氮微空心阴极放电（N2-MHCD）等离子体灭活大肠杆菌（E. coli）。结果表明，空气- sdbd等离子体在30秒内使大肠杆菌减少了6倍，而其他三种源即使在120秒后也未能实现完全失活。空气- sdbd产生的水中H2O2、NO2−和NO3−浓度比空气- tsd和He-APPJ高2 ~ 3倍。空气- sdbd产生的O3浓度是N2-MHCD的4倍以上。为了进一步探讨四种来源产生的ron与大肠杆菌灭活之间的关系，我们分析了四种来源对12种代表性氨基酸的影响。液相色谱-质谱（LC-MS）分析表明，四种来源处理后，苯丙氨酸（Phe）、蛋氨酸（Met）、色氨酸（Trp）、酪氨酸（Tyr）、谷氨酸（Glu）、赖氨酸（Lys）、组氨酸（His）、精氨酸（Arg）和半胱氨酸（Cys）等氨基酸均发生了明显的氧化。与其他三种来源相比，Air-SDBD处理后Met、Trp和Cys的降低更为明显。我们的研究结果表明，大肠杆菌的失活与ron修饰氨基酸之间存在很强的相关性。

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来源期刊

Plasma Chemistry and Plasma Processing 工程技术-工程：化工

CiteScore

5.90

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Publishing original papers on fundamental and applied research in plasma chemistry and plasma processing, the scope of this journal includes processing plasmas ranging from non-thermal plasmas to thermal plasmas, and fundamental plasma studies as well as studies of specific plasma applications. Such applications include but are not limited to plasma catalysis, environmental processing including treatment of liquids and gases, biological applications of plasmas including plasma medicine and agriculture, surface modification and deposition, powder and nanostructure synthesis, energy applications including plasma combustion and reforming, resource recovery, coupling of plasmas and electrochemistry, and plasma etching. Studies of chemical kinetics in plasmas, and the interactions of plasmas with surfaces are also solicited. It is essential that submissions include substantial consideration of the role of the plasma, for example, the relevant plasma chemistry, plasma physics or plasma–surface interactions; manuscripts that consider solely the properties of materials or substances processed using a plasma are not within the journal’s scope.