Stimulated Production of Heat Stable Antifungal Factor by Plasma-Activated Water

IF 2.5 3区物理与天体物理 Q3 ENGINEERING, CHEMICAL

Plasma Chemistry and Plasma Processing Pub Date : 2025-07-17 DOI:10.1007/s11090-025-10581-0

Matthew R. Winburn, Kyle L. Schuelke, Amanda Lynn Miller, Pinky Chowdhury, Liangcheng Du, Chin Li Cheung

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引用次数: 0

Abstract

Plasma-activated water (PAW) produced by different methods has been intensively studied for its biomedical applications due to the antimicrobial effects of reactive oxygen and nitrogen species within. While many of these studies focus on the effects of PAW on bacterial death, other bacterial responses to PAW are seldom assessed. Herein, we report an evaluation of PAW produced by a falling-film plasma reactor (FFPR) on the growth of Lysobacter enzymogenes and its biosynthesis of the natural products - heat stable antifungal factor (HSAF) and its analogs. An FFPR setup was demonstrated to effectively create plasma-treated deionized water under atmospheric conditions for the generation of PAW. These PAW samples were shown to contain nitrite, nitrate, and hydrogen peroxide of concentrations that were dependent on the plasma activation time. Short periods of PAW activation caused L. enzymogenes to significantly increase the production of HSAF, its analogs, and total cell growth. The PAW produced with a longer plasma activation period had higher concentrations of nitrate and hydrogen peroxide, and was found to have decreased growth in L. enzymogenes. These results shed new light that PAW can also be used to stimulate the production of natural products. Furthermore, the activation period of PAW can be optimized to stimulate either an increase in the total HSAF yield or HSAF yield per optical density unit in a cell culture.

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等离子体活化水刺激热稳定抗真菌因子的产生

由于等离子体活性水中活性氧和活性氮的抗菌作用，不同方法生产的等离子体活性水（PAW）在生物医学上的应用得到了广泛的研究。虽然这些研究大多集中在PAW对细菌死亡的影响上，但很少评估其他细菌对PAW的反应。在此，我们报道了用降膜等离子体反应器（FFPR）生产的PAW对溶菌酶原的生长及其天然产物-热稳定抗真菌因子（HSAF）及其类似物的生物合成的影响。FFPR装置被证明可以在大气条件下有效地产生等离子体处理的去离子水，用于产生PAW。这些PAW样品被证明含有亚硝酸盐、硝酸盐和过氧化氢，其浓度取决于等离子体激活时间。短时间的PAW激活导致L.酶原菌显著增加HSAF及其类似物的产量和细胞总生长。血浆活化时间较长的木瓜含有较高的硝酸盐和过氧化氢浓度，并抑制了L.酶原菌的生长。这些结果揭示了PAW也可以用来刺激天然产物的产生。此外，可以优化PAW的激活周期，以刺激总HSAF产量或细胞培养中每光密度单位HSAF产量的增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasma Chemistry and Plasma Processing 工程技术-工程：化工

CiteScore

5.90

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Publishing original papers on fundamental and applied research in plasma chemistry and plasma processing, the scope of this journal includes processing plasmas ranging from non-thermal plasmas to thermal plasmas, and fundamental plasma studies as well as studies of specific plasma applications. Such applications include but are not limited to plasma catalysis, environmental processing including treatment of liquids and gases, biological applications of plasmas including plasma medicine and agriculture, surface modification and deposition, powder and nanostructure synthesis, energy applications including plasma combustion and reforming, resource recovery, coupling of plasmas and electrochemistry, and plasma etching. Studies of chemical kinetics in plasmas, and the interactions of plasmas with surfaces are also solicited. It is essential that submissions include substantial consideration of the role of the plasma, for example, the relevant plasma chemistry, plasma physics or plasma–surface interactions; manuscripts that consider solely the properties of materials or substances processed using a plasma are not within the journal’s scope.