The study on the design and regulatory effects of synthetic communities of endophytic actinomycetes of Salvia miltiorrhiza Bunge

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2025-08-08 DOI:10.1186/s40538-025-00815-w

Changwen Zheng, Wencheng Zhao, Yurui Wu, Hongmei Jia, Guiqi Han, Dongmei He, Hai Wang, Zhuyun Yan

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引用次数: 0

Abstract

Background

Microorganisms can confer or enhance various ecological adaptation functions of host plants, offering wide-ranging applications in the cultivation and production of medicinal plants. While root-associated beneficial actinomycetes are commonly used in biological control, their effects on plant growth and development have been rarely reported. This study focuses on a total of 13 beneficial endophytic actinomycetes in the roots of Salvia miltiorrhiza Bunge. Under different synthetic community (SynCom) construction principles, we constructed five synthetic communities based on functions and relationships to investigate their effects on growth, nitrogen metabolism, phosphorus metabolism, iron metabolism, resistance, and active components of S. miltiorrhiza.

Results

Synthetic communities 3 and 4 composed of functionally complementary strains promoted growth more effectively, synthetic communities 1 and 3 composed of distantly related strains increased total phenolic acid content, and synthetic community 5 that composed solely of indole-3-acetic acid (IAA)-producing strains not only promoted growth but also enhanced the active component content. Genome analysis revealed that synthetic communities with terpenes biosynthetic gene clusters promoted S. miltiorrhiza growth.

Conclusions

Our results suggested synthetic community design should consider strain relationships, plant growth-promoting (PGP) traits, and functional genes. This study introduces novel insights into synthetic community design.

Graphical Abstract

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丹参内生放线菌合成群落的设计及调控作用研究

微生物可以赋予或增强寄主植物的多种生态适应功能，在药用植物的栽培和生产中有着广泛的应用。虽然根相关的有益放线菌通常用于生物防治，但它们对植物生长发育的影响很少报道。本研究对丹参根中13种有益内生放线菌进行了研究。在不同的合成群落（SynCom）构建原则下，以功能和关系为基础，构建了5个合成群落，探讨其对丹参生长、氮代谢、磷代谢、铁代谢、抗性和活性成分的影响。结果由功能互补菌株组成的合成群落3和4能更有效地促进生长，由远亲菌株组成的合成群落1和3能提高总酚酸含量，由产吲哚-3-乙酸（IAA）菌株组成的合成群落5不仅能促进生长，还能提高活性成分含量。基因组分析表明，含有萜类生物合成基因簇的合成群落促进了丹参的生长。结论综合群落设计应考虑品系关系、植物促生长性状和功能基因等因素。本研究引入了合成社区设计的新见解。图形抽象

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.