Identification of antioxidant peptides from a bioactive peptide database PWDB of walnut family protein hydrolysates

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-09-26 DOI:10.1016/j.foodchem.2025.146548

Tianle Zhang , Dingwei Zheng , Siyuan Qian , Mingjun Du , Caiyun Li , Zhichao Sun , Li Lü , Jianqin Huang , Liangsheng Zhang , Zhengjia Wang

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Abstract

This study established Walnut family Peptide DataBase (PWDB), a comprehensive peptide database containing 3894 high-confidence peptides derived from walnut family hydrolysates through LC-MS/MS (<3 kDa) coupled with advanced bioinformatic validation. Screening of PWDB identified three core antioxidant peptides—WDPFE, FDPFP, and WDPNY—with significant free radical scavenging abilities and protective effects against oxidative stress. Molecular docking simulation revealed strong interactions between these peptides and key antioxidant receptors Kelch-like ECH-associated protein 1 (Keap1), Myeloperoxidase (MPO), and Xanthine Oxidase (XO), supporting their multi-target antioxidative potential. In vitro assays using Caco-2 cells models confirmed their ability to mitigate oxidative damage in enhancing cellular viability and reducing reactive oxygen species (ROS). This study highlights walnut family as a sustainable source of bioactive peptides, providing a valuable resource for developing natural antioxidants for functional foods.

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从核桃家族蛋白水解物的生物活性肽数据库PWDB中鉴定抗氧化肽

本研究通过LC-MS/MS （<3 kDa）结合先进的生物信息学验证，建立了核桃家族肽数据库（PWDB），该数据库包含3894个高可信度的核桃家族水解产物肽。PWDB的筛选鉴定出三个核心抗氧化肽- wdpfe、FDPFP和wdpny -具有显著的自由基清除能力和抗氧化应激保护作用。分子对接模拟显示，这些肽与关键抗氧化受体Kelch-like ECH-associated protein 1 （Keap1）、髓过氧化物酶（MPO）和黄嘌呤氧化酶（XO）之间存在强相互作用，支持其多靶点抗氧化潜力。使用Caco-2细胞模型的体外实验证实了它们能够减轻氧化损伤，提高细胞活力和减少活性氧（ROS）。本研究强调核桃家族作为生物活性肽的可持续来源，为开发功能性食品的天然抗氧化剂提供了宝贵的资源。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.