Comprehensive evaluation of heat response in commonly cultivated barley (Hordeum vulgare L.) landraces of Tunisia

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Rania Djemal, Chantal Ebel, Ikram Zaidi, Mohamed Najib Saidi, Agostino Fricano, Moez Hanin
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引用次数: 0

Abstract

Mediterranean cereal crop production is expected to be severely affected by heat waves. Our knowledge on the physiological and molecular responses of barley (Hordeum vulgare L) to elevated temperature is particularly scarce. Here, we performed a multivariate analysis to evaluate the response to heat stress of the most common barley landraces (Arturio, Kounouz, Farandole, Arkansas, Manel, Mercure, Sextine, Imen, Rihane) grown in Tunisia. After an exposure of these genotypes during the vegetative growth stage to high temperature (32 °C) for 5 days, measurements of growth parameters along with physiological, biochemical and molecular analyses were performed. Our results showed a large variation among these genotypes in root length, plant height, number of leaves, relative water content (RWC), electrolyte leakage (EL), the contents of chlorophyll, proline, soluble sugars and antioxidants enzymes activities like CAT and SOD. Principal component analysis (PCA) and heatmap analysis showed that Kounouz and other landraces including Imen, Rihane, Manel, and Mercure are predominantly associated with heat stress tolerance parameters like CAT, SOD, RWC, and soluble sugars activities. However, Farandole, Arturio, and Arkansas are rather associated with the oxidative stress marker H2O2 and MDA. Kounouz and Farandole appear as contrasting varieties with heat stress tolerant and sensitive traits respectively, as it was confirmed by evaluating the oxidative stress status. Remarkably, expression analysis of stress-responsive genes in the two contrasting genotypes under heat stress, showed higher induction of most of these genes in Kounouz compared to Farandole. Altogether, this study strongly suggests that Kounouz is among the most heat-tolerant genotypes and should be included in future breeding programs for a better adaptation of barley to heat waves.

突尼斯普通栽培大麦地方品种热响应的综合评价
预计地中海谷物作物的生产将受到热浪的严重影响。我们对大麦(Hordeum vulgare L)对高温的生理和分子反应的了解尤其缺乏。在这里,我们进行了多变量分析,以评估在突尼斯种植的最常见的大麦地方品种(Arturio, Kounouz, Farandole, Arkansas, Manel, Mercure, Sextine, Imen, Rihane)对热胁迫的反应。在营养生长期将这些基因型暴露在高温(32°C)下5天后,进行生长参数测量以及生理、生化和分子分析。结果表明,这些基因型在根长、株高、叶片数、相对含水量(RWC)、电解质泄漏(EL)、叶绿素含量、脯氨酸含量、可溶性糖含量以及CAT和SOD等抗氧化酶活性等方面存在较大差异。主成分分析(PCA)和热图分析表明,Kounouz和Imen、Rihane、Manel和Mercure等地方品种与CAT、SOD、RWC和可溶性糖活性等耐热性参数相关。然而,Farandole, Arturio和Arkansas与氧化应激标志物H2O2和MDA相关。Kounouz和Farandole分别表现为耐热和敏感性状的对照品种,这一点通过评价氧化应激状态得到了证实。值得注意的是,在热胁迫下,两种不同基因型的胁迫响应基因的表达分析显示,与Farandole相比,Kounouz对大多数这些基因的诱导程度更高。总之,这项研究强烈表明,Kounouz是最耐热的基因型之一,应该包括在未来的育种计划中,以更好地适应大麦对热浪。
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来源期刊
Acta Physiologiae Plantarum
Acta Physiologiae Plantarum 生物-植物科学
CiteScore
5.10
自引率
3.80%
发文量
125
审稿时长
3.1 months
期刊介绍: Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.
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