Determination of gene expression of some essential oil components and some genes responsible for the synthesis of these components in Mentha species harvested in different phenological periods

IF 2.2 4区 生物学 Q2 PLANT SCIENCES
Civan Çelik, Ümmü Tuğlu, İsa Telci, Yaşar Karakurt, Temel Özek, Gülmira Özek
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引用次数: 0

Abstract

Peppermint (Mentha × piperita) is widely recognized as a significant medicinal plant worldwide. This study aimed to evaluate the gene expression levels of key genes involved in essential oil biosynthesis—limonene synthase (ls), limon-3-hydroxylase (l3oh), trans-isopiperitenol dehydrogenase (ipd), isopiperitenone reductase (ipr), menthol dehydrogenase (mdeh), and menthofuran synthase (mfs)—as well as the changes in various essential oil components across phenological stages in selected mint samples. The results revealed that the expression levels of genes associated with terpene biosynthesis varied among peppermint varieties. Additionally, seasonal variations were observed in the essential oil components of the studied varieties. While changes in the main chemical constituents were not always consistent with gene expression levels, some terpenes present in lower concentrations exhibited patterns that aligned with gene activity. Moreover, varieties displaying higher seasonal averages of gene expression levels were found to have higher overall essential oil content. This suggests that the genes studied play a broader role in the synthesis of terpene groups rather than being limited to the regulation of specific primary constituents. These findings provide important insights into the genetic and biochemical mechanisms regulating essential oil composition in mint. Such knowledge can contribute to the development of targeted breeding and cultivation strategies aimed at enhancing the medicinal and commercial value of peppermint.

不同物候期采收的薄荷精油成分及其合成相关基因表达的测定
薄荷(Mentha × piperita)是世界上公认的重要药用植物。本研究旨在评价精油生物合成关键基因——柠檬烯合成酶(ls)、柠檬-3-羟化酶(l3oh)、反式异戊二烯醇脱氢酶(ipd)、异戊二烯酮还原酶(ipr)、薄荷醇脱氢酶(mdeh)和薄荷呋喃合成酶(mfs)在不同物候阶段的表达水平以及不同精油成分的变化。结果表明,不同薄荷品种间萜类生物合成相关基因的表达水平存在差异。此外,所研究品种的精油成分也存在季节变化。虽然主要化学成分的变化并不总是与基因表达水平一致,但一些较低浓度的萜烯表现出与基因活性一致的模式。此外,基因表达水平季节性平均值较高的品种总体精油含量较高。这表明所研究的基因在萜烯基团的合成中发挥更广泛的作用,而不是局限于对特定主要成分的调节。这些发现对薄荷精油成分的遗传和生化调控机制提供了重要的见解。这些知识有助于制定有针对性的育种和栽培策略,旨在提高薄荷的药用和商业价值。
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来源期刊
Acta Physiologiae Plantarum
Acta Physiologiae Plantarum 生物-植物科学
CiteScore
5.10
自引率
3.80%
发文量
125
审稿时长
3.1 months
期刊介绍: Acta Physiologiae Plantarum is an international journal established in 1978 that publishes peer-reviewed articles on all aspects of plant physiology. The coverage ranges across this research field at various levels of biological organization, from relevant aspects in molecular and cell biology to biochemistry. The coverage is global in scope, offering articles of interest from experts around the world. The range of topics includes measuring effects of environmental pollution on crop species; analysis of genomic organization; effects of drought and climatic conditions on plants; studies of photosynthesis in ornamental plants, and more.
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