Immobilized pigeon pea urease on chitosan-PEG biocomposite for concurrent urea sensing in milk and blood samples

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-09-25 DOI:10.1016/j.foodchem.2025.146509

Prince Kumar, Aditya Ghosal, Arvind M. Kayastha

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引用次数: 0

Abstract

The ubiquitous hydrolytic enzyme urease converts urea into ammonia and carbon dioxide, making it an essential indicator for dairy product quality and kidney health. We developed a biocomposite sensor to meet this diagnostic necessity. This innovation strategically incorporates pigeon pea seed urease into chitosan-PEG. We verified our predicted model with a 91.30 % immobilization efficiency via our thorough process. The immobilized urease showed strong catalytic activity, with a V_max of 3194 μmol/min/mg and a K_m of 0.373 mM. Optimal activity was observed at 90 °C and pH 8.0. Additionally, it exhibited exceptional operational stability, maintaining 56 % activity over 200 days at 4 °C and 60 % reusability over 11 cycles. The sensor showed a wide linear detection range (5–400 mg/dL) with high sensitivity (LOD 3.10 (±0.155) mg/dL, LOQ 10.36 (±0.510) mg/dL). Its high selectivity (K_sel < 0.08) ensures precise urea detection in complex milk and blood matrices. This stable and reproducible biocomposite is a breakthrough in dairy and healthcare analytical tools.

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壳聚糖-聚乙二醇生物复合材料固定化鸽豌豆脲酶在牛奶和血液样品中同时检测尿素

无处不在的水解酶脲酶将尿素转化为氨和二氧化碳，使其成为乳制品质量和肾脏健康的重要指标。我们开发了一种生物复合传感器来满足这种诊断需求。这一创新战略性地将鸽豌豆种子脲酶融入壳聚糖聚乙二醇中。结果表明，该模型的固定效率为91.30 %。固定化脲酶表现出较强的催化活性，Vmax为3194 μmol/min/mg， Km为0.373 mM。在90 °C和pH 8.0条件下，活性最佳。此外，它还表现出了出色的运行稳定性，在4 °C下，在200 天内保持了56% %的活性，在11 次循环中保持了60% %的可重用性。该传感器线性检测范围宽（5 ~ 400 mg/dL），灵敏度高（LOD 3.10（±0.155）mg/dL, LOQ 10.36（±0.510）mg/dL）。其高选择性（Ksel <； 0.08）确保在复杂的牛奶和血液基质中精确检测尿素。这种稳定和可重复的生物复合材料是乳制品和医疗保健分析工具的突破。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.