Dual-readout lateral flow immunoassay for detection of amphenicols using intensified colorimetric–fluorescent carbon dots nanocomposite

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-09-26 DOI:10.1016/j.foodchem.2025.146398

Hongfang Li, Wenqing Xu, Yina Zhu, Hongxing Wei, Xiaoyue Liu, Lin Li, Guoliang Li

{"title":"Dual-readout lateral flow immunoassay for detection of amphenicols using intensified colorimetric–fluorescent carbon dots nanocomposite","authors":"Hongfang Li, Wenqing Xu, Yina Zhu, Hongxing Wei, Xiaoyue Liu, Lin Li, Guoliang Li","doi":"10.1016/j.foodchem.2025.146398","DOIUrl":null,"url":null,"abstract":"Conventional lateral flow immunoassays (LFIA) employing gold nanoparticles as probes suffer from insufficient sensitivity and poor accuracy in detecting amphenicols in food. Developing a dual-readout LFIA with fluorescence and colorimetric signals can significantly enhance its performance. Herein, fluorescent carbon dots were encapsulated into SiO2 nanospheres to obtain the SiO2/CDs nanocomposites with a quantum yield of 81.26 %. The encapsulation enhances the signal intensity and prevents fluorescence quenching of carbon dots. Surface modification of SiO2/CDs with polydopamine (PDA) enables efficient antibody conjugation. The strong light absorption capacity of PDA also endowed the nanocomposites with good colorimetric signals. The SiO2/CDs@PDA-based LFIA showed 5-fold and 71-fold improvements in chloramphenicol detection using colorimetric and fluorescent modes, compared to conventional LFIA. The quantitative detection limits were 1.03–1.54 ng/g and 0.1–0.78 ng/g in colorimetric and fluorescent mode for amphenicols in chicken. The proposed LFIA offers a rapid, accurate, and reliable method for food safety monitoring.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"319 1","pages":""},"PeriodicalIF":9.8000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.foodchem.2025.146398","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Conventional lateral flow immunoassays (LFIA) employing gold nanoparticles as probes suffer from insufficient sensitivity and poor accuracy in detecting amphenicols in food. Developing a dual-readout LFIA with fluorescence and colorimetric signals can significantly enhance its performance. Herein, fluorescent carbon dots were encapsulated into SiO₂ nanospheres to obtain the SiO₂/CDs nanocomposites with a quantum yield of 81.26 %. The encapsulation enhances the signal intensity and prevents fluorescence quenching of carbon dots. Surface modification of SiO₂/CDs with polydopamine (PDA) enables efficient antibody conjugation. The strong light absorption capacity of PDA also endowed the nanocomposites with good colorimetric signals. The SiO₂/CDs@PDA-based LFIA showed 5-fold and 71-fold improvements in chloramphenicol detection using colorimetric and fluorescent modes, compared to conventional LFIA. The quantitative detection limits were 1.03–1.54 ng/g and 0.1–0.78 ng/g in colorimetric and fluorescent mode for amphenicols in chicken. The proposed LFIA offers a rapid, accurate, and reliable method for food safety monitoring.

Abstract Image

查看原文本刊更多论文

强化比色-荧光碳点纳米复合材料双读数横向流动免疫分析法检测霉素

传统的以金纳米颗粒为探针的横向流动免疫分析法（LFIA）在检测食品中的霉素时存在灵敏度不足和准确性差的问题。开发具有荧光和比色信号的双读出LFIA可以显著提高其性能。将荧光碳点包裹在SiO2纳米球中，得到量子产率为81.26 %的SiO2/CDs纳米复合材料。这种封装提高了碳点的信号强度，防止了碳点的荧光猝灭。用聚多巴胺（PDA）修饰SiO2/CDs表面，实现了高效的抗体偶联。PDA的强光吸收能力也赋予了纳米复合材料良好的比色信号。与传统的LFIA相比，SiO2/CDs@PDA-based LFIA在比色和荧光模式下对氯霉素的检测分别提高了5倍和71倍。鸡体内氨霉素的定量检出限分别为1.03 ~ 1.54 ng/g和0.1 ~ 0.78 ng/g。提出的LFIA为食品安全监测提供了一种快速、准确、可靠的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.