Characterization of propolis extracts from the Tolima region in Colombia: a study of their composition, antimicrobial activity, and cytotoxicity.

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-25 DOI:10.1002/jsfa.70223

July Alexandra Hernandez-Lopez, Brandhon Francisco Flores-Ibarra, Eduart Gutierrez-Pineda, Sergio E Moya, Guillermo Salamanca Grosso, Cristian C Villa

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引用次数: 0

Abstract

Background: Propolis is a complex resinous material collected by bees from plant sources and known for its rich content in bioactive phenolic compounds, including flavonoids and aromatic acids. Its chemical composition and biological activity are highly influenced by the botanical and environmental conditions surrounding the beehives. In Colombia, limited studies have addressed the chemical diversity and therapeutic potential of propolis from regions with high altitudinal variation such as Tolima.

Results: In this study, three propolis ethanolic extracts (PrEE1, PrEE2, PrEE3) were obtained from hives located at different altitudes in Tolima, Colombia (1250, 1100, and 900 m above sea level (a.s.l.), respectively). Samples were analyzed for total phenolic content (TPC), total flavonoid content (TFC), antioxidant capacity (DPPH, ABTS, FRAP), antimicrobial activity (disk diffusion and minimum inhibitory concentration), and cytotoxicity in A549, WI-38 and NIH-3 T3 cell lines. Ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis revealed a distinct phytochemical profile for each extract. PrEE1 had the highest TPC (130.2 g GAE kg^-1) and TFC (6.70 g QE kg^-1), and strong antioxidant activity with DPPH scavenging of 89.2%. It also exhibited potent antimicrobial activity against Staphylococcus aureus and Candida albicans, and selective cytotoxicity toward A549 lung cancer cells. In contrast, PrEE3 had lower phenolic content but contained unique polyprenylated benzophenones.

Conclusion: The results indicate that altitude significantly influences the chemical and biological properties of Colombian propolis. Propolis from higher altitudes, such as PrEE1, is a promising natural source of multifunctional bioactive compounds with antioxidant, antimicrobial, and anticancer potential for pharmaceutical and nutraceutical applications. © 2025 Society of Chemical Industry.

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哥伦比亚托利马地区蜂胶提取物的表征：其组成、抗菌活性和细胞毒性的研究。

背景：蜂胶是蜜蜂从植物中收集的一种复杂的树脂材料，以其富含生物活性酚类化合物而闻名，包括类黄酮和芳香酸。其化学成分和生物活性受蜂箱周围的植物和环境条件的高度影响。在哥伦比亚，有限的研究解决了来自托利马等高海拔变化地区蜂胶的化学多样性和治疗潜力。结果：从哥伦比亚Tolima不同海拔（海拔分别为1250、1100和900 m）的蜂箱中获得了3种蜂胶乙醇提取物（PrEE1、PrEE2、PrEE3）。对A549、WI-38和NIH-3 T3细胞株的总酚含量（TPC）、总黄酮含量（TFC）、抗氧化能力（DPPH、ABTS、FRAP）、抑菌活性（盘扩散和最小抑菌浓度）和细胞毒性进行分析。超高效液相色谱-质谱（UHPLC-MS）分析显示每种提取物具有不同的植物化学特征。PrEE1具有最高的TPC （130.2 g GAE kg-1）和TFC (6.70 g QE kg-1)，具有较强的抗氧化活性，对DPPH的清除率为89.2%。对金黄色葡萄球菌和白色念珠菌也有较强的抑菌活性，对A549肺癌细胞有选择性的细胞毒性。相比之下，PrEE3的酚类含量较低，但含有独特的聚戊烯基二苯甲酮。结论：海拔对哥伦比亚蜂胶的化学生物学特性有显著影响。来自高海拔地区的蜂胶，如PrEE1，是一种具有抗氧化、抗菌和抗癌潜力的多功能生物活性化合物的天然来源，在制药和营养保健方面具有应用前景。©2025化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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