Eco-friendly extraction of collagen from beluga sturgeon skin using ultrasound-assisted deep eutectic solvents: efficiency and characterization.

IF 8.2 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry: X Pub Date : 2025-09-03 eCollection Date: 2025-08-01 DOI:10.1016/j.fochx.2025.102988

Elahe Taghitabar Malekshah, Masoud Rezaei, Shahab Naghdi, Reza Tahergorabi

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引用次数: 0

Abstract

The current study aims to extract collagen from the skin of beluga sturgeon (Huso huso) using deep eutectic solvents combined with ultrasound as a green recovery method. It evaluates both the yield of the extraction process and the structural and physicochemical properties of the extracted collagen. The extraction utilized deep eutectic solvents, including choline chloride-glycerol, choline chloride-urea, and choline chloride-acetic acid, all at a molar ratio of 1:2. Among these, the choline chloride-acetic acid solvent yielded the highest extraction rate at 20.28 % and was selected for further ultrasound treatments lasting 5, 10, 15, and 20 min, with a power setting of 400 W. Ultrasound treatment significantly enhanced collagen extraction yield, with a 10-min treatment achieving the highest efficiency at 24 %. SDS-PAGE analysis confirmed the presence of type I collagen, indicating no structural changes during extraction. FTIR analysis showed that the triple helical structure of ultrasound-treated collagen was preserved throughout the process. Longer ultrasound treatment durations improved collagen purification, resulting in higher protein and total amino acid content. Additionally, ultrasound application enhanced thermal stability, water holding capacity, and gel formation power, with peak values observed during a 15-min treatment. In summary, extracting collagen from beluga skin using deep eutectic solvents in combination with ultrasound is an effective method for enhancing both yield and functionality of the resulting collagen.

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利用超声波辅助的深层共熔溶剂从白鲟皮肤中环保提取胶原蛋白：效率和特性。

本研究旨在利用深共熔溶剂结合超声波作为绿色回收方法，从白鲟（Huso Huso）皮肤中提取胶原蛋白。它评估了提取过程的收率以及提取的胶原蛋白的结构和物理化学性质。采用氯化胆碱-甘油、氯化胆碱-尿素、氯化胆碱-乙酸等深共晶溶剂，摩尔比均为1:2。其中氯胆碱-乙酸溶剂提取率最高，为20.28%，超声处理时间分别为5、10、15、20 min，功率设置为400 W。超声处理显著提高胶原蛋白提取率，10分钟的处理达到24%的最高效率。SDS-PAGE分析证实了I型胶原蛋白的存在，表明提取过程中没有结构变化。FTIR分析表明，超声处理的胶原蛋白在整个过程中保持了三螺旋结构。较长的超声治疗持续时间改善胶原蛋白纯化，导致更高的蛋白质和总氨基酸含量。此外，超声波应用增强了热稳定性、保水能力和凝胶形成能力，在处理15分钟时观察到峰值。综上所述，利用深层共熔溶剂结合超声波从白鲸皮肤中提取胶原蛋白是一种有效的方法，可以提高所得胶原蛋白的收率和功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Chemistry: X CHEMISTRY, APPLIED-

CiteScore

4.90

自引率

6.60%

发文量

315

审稿时长

55 days

期刊介绍： Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.