Effects of flickering light stimulation on retinal blood flow and full-field electroretinogram in mice.

IF 2.9 4区医学 Q2 OPHTHALMOLOGY

Documenta Ophthalmologica Pub Date : 2025-09-25 DOI:10.1007/s10633-025-10049-8

Milan Rai, Yamunadevi Lakshmanan, Kai Yip Choi, Henry Ho-Lung Chan

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引用次数: 0

Abstract

Purpose: To investigate the effects of brief flickering light stimulation (FLS) on retinal electrophysiology and retinal blood flow (RBF) in normal C57BL6J mice.

Methods: RBF and full-field electroretinography (ffERG) were measured before and after a 60 second FLS (12 Hz, 0.1 cd·s/m²) in a cohort of 8-12-weeks old C57BL6J mice (n=10) under anaesthetic and light-adapted conditions. A separate set of age-matched mice (n=9) underwent RBF and ffERG measurements before and after steady light stimulation (SLS) at 1 cd/m² under similar conditions. The changes in RBF (arterial and venous flow) as well as the amplitudes and implicit times of the a-wave, b-wave, oscillatory potentials (OPs), and photopic negative response (PhNR) were analyzed.

Results: FLS significantly increased both arterial (p=0.003) and venous (p=0.018) blood flow as well as b-wave amplitudes (p=0.017) compared to SLS, which did not have any significant changes in either RBF or ERG. However, no significant differences were found in other ffERG responses (amplitudes and implicit times of a-wave, OPs, and PhNR, as well as b-wave implicit time) between the two groups after light stimulation. An increase in b-wave amplitude was positively associated with an increase in both arterial (r=0.655, p=0.040) and venous blood flow (r=0.638, p=0.047) in the FLS group.

Conclusions: Our results suggest that transient FLS not only increases RBF but also enhances electro-retinal responses of the middle retinal layer, as shown by ffERG, thus demonstrating its substantial effects on both the vascular and neuronal components of retinal neurovascular coupling in mice.

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闪烁光刺激对小鼠视网膜血流及全场视网膜电图的影响。

目的：探讨短暂闪烁光刺激（FLS）对正常C57BL6J小鼠视网膜电生理及视网膜血流（RBF）的影响。方法：在麻醉和光适应条件下，8-12周龄C57BL6J小鼠（n=10）在60秒（12 Hz, 0.1 cd·s/m2）快闪前和快闪后测量RBF和全视场视网膜电图（ffERG）。另一组年龄匹配的小鼠（n=9）在类似条件下，在1 cd/m2的稳定光刺激（SLS）前后分别测量RBF和ffERG。分析大鼠动脉和静脉血流的变化，以及a波、b波、振荡电位（OPs）和光负反应（PhNR）的振幅和隐次数。结果：与SLS相比，FLS显著增加了动脉血流量（p=0.003）和静脉血流量（p=0.018）以及b波振幅（p=0.017），而SLS对RBF和ERG均无显著变化。然而，光刺激后两组之间的其他ffERG反应（a波、OPs和PhNR的振幅和隐式时间，以及b波隐式时间）无显著差异。FLS组b波振幅的增加与动脉血流（r=0.655, p=0.040）和静脉血流量（r=0.638, p=0.047）的增加呈正相关。结论：我们的研究结果表明，瞬态FLS不仅增加了RBF，而且增强了视网膜中间层的电视网膜反应，如ffERG所示，从而表明其对小鼠视网膜神经血管耦合的血管和神经元成分都有实质性的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Documenta Ophthalmologica 医学-眼科学

CiteScore

3.50

自引率

21.40%

发文量

审稿时长

>12 weeks

期刊介绍： Documenta Ophthalmologica is an official publication of the International Society for Clinical Electrophysiology of Vision. The purpose of the journal is to promote the understanding and application of clinical electrophysiology of vision. Documenta Ophthalmologica will publish reviews, research articles, technical notes, brief reports and case studies which inform the readers about basic and clinical sciences related to visual electrodiagnosis and means to improve diagnosis and clinical management of patients using visual electrophysiology. Studies may involve animals or humans. In either case appropriate care must be taken to follow the Declaration of Helsinki for human subject or appropriate humane standards of animal care (e.g., the ARVO standards on Animal Care and Use).