An Automated Multi-Sample Digital LAMP Platform for Quantitative Detection of SDC2 Methylation in Colorectal Cancer.

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-09-24 DOI:10.1021/acs.analchem.5c03244

Hao He,Yanzhao Wu,Wei Lai,Lin Zhou,Zhirui Liu,Nan Rong,Chunxiong Luo,Chanqiong Zhang,Wei Yang

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引用次数: 0

Abstract

DNA methylation of the SDC2 gene has emerged as a critical biomarker for early detection of colorectal cancer (CRC). However, effective clinical application demands analytical platforms that offer enhanced accuracy, speed, and sample high-throughput capacity. In this study, we present an automated multisample digital loop-mediated isothermal amplification (dLAMP) platform designed to meet these requirements. Our platform integrates a dual-volume microfluidic chip capable of analyzing 4 or 8 samples in parallel, significantly enhancing sample throughput and efficiency. Integrated with automatic sample injection and advanced image processing, the platform achieves streamlined, multistep analysis of SDC2 methylation levels. We designed methylation-specific (MS) and unmethylation-specific (UMS) LAMP primers with high specificity. Validation using CRC cell lines (HCT116 and Caco-2) and clinical tissue samples demonstrated robust performance with high sensitivity, specificity and reproducibility. The results showed elevated SDC2 methylation was detected in tumor tissues across all CRC stages. This integrated automated dLAMP platform provides a robust, multisample high-throughput tool for methylation-based diagnostics and holds strong potential for noninvasive early cancer screening and clinical biomarker applications.

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结直肠癌中SDC2甲基化的自动多样本数字LAMP定量检测平台

SDC2基因的DNA甲基化已成为结直肠癌（CRC）早期检测的关键生物标志物。然而，有效的临床应用需要分析平台提供更高的准确性、速度和样品高通量能力。在这项研究中，我们提出了一个自动多样本数字环路介导等温扩增（dLAMP）平台，旨在满足这些要求。我们的平台集成了双体积微流控芯片，能够同时分析4或8个样品，显著提高了样品的吞吐量和效率。该平台集成了自动进样和先进的图像处理，实现了SDC2甲基化水平的流线型多步分析。我们设计了高特异性的甲基化特异性（MS）和非甲基化特异性（UMS） LAMP引物。使用结直肠癌细胞系（HCT116和Caco-2）和临床组织样本进行验证，显示出具有高灵敏度、特异性和可重复性的强大性能。结果显示，在所有CRC分期的肿瘤组织中均检测到SDC2甲基化升高。这种集成的自动化dLAMP平台为基于甲基化的诊断提供了强大的多样本高通量工具，并在无创早期癌症筛查和临床生物标志物应用方面具有强大的潜力。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.