Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial-mesenchymal transition, and metastasis in breast cancer.

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-22 DOI:10.1002/jsfa.70175

Asad Ur Rahman, Naveed Ullah Khan, Abdul Basit, Muhammad Khan, Jiang Ni, Pharkphoom Panichayupakaranant

{"title":"Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial-mesenchymal transition, and metastasis in breast cancer.","authors":"Asad Ur Rahman, Naveed Ullah Khan, Abdul Basit, Muhammad Khan, Jiang Ni, Pharkphoom Panichayupakaranant","doi":"10.1002/jsfa.70175","DOIUrl":null,"url":null,"abstract":"Background: Breast cancer (BC) is a life-threatening disease, with approximately 60% of cases progressing to breast cancer lung metastasis (BCLM). Chamuangone, a key bioactive constituent derived from the leaves of the Thai plant Garcinia cowa Roxb., has demonstrated significant potential as an anticancer agent. To evaluate its anticancer effects, chamuangone was purified from the leaves and assessed through in silico, in vitro, and in vivo studies using a mouse model of BC.Results: The results revealed that chamuangone (10 mg L-1) inhibited cancer cell proliferation by inducing G0/G1 phase arrest and apoptosis in 4T1-luciferase (4T1-luc) and Michigan Cancer Foundation-7 (MCF-7) cells. It also modulated the levels of key apoptotic markers, including B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and caspase-3. Similarly, in vivo studies demonstrated that chamuangone significantly (P < 0.05) inhibited breast tumor growth and lung metastasis at a dose of 100 mg kg⁻¹ body weight, in comparison with the standard drug doxorubicin (5 mg kg⁻¹ body weight) in mice. Immunohistochemical analysis further revealed that chamuangone modulated several BCLM-associated markers, including estrogen receptor (ER), progesterone receptor (PR), Ki-67 antigen (Ki-67), cluster of differentiation 44 (CD44), E-cadherin, N-cadherin, vascular endothelial growth factor A (VEGFA), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), in both breast tumors and lung tissues. These effects contributed to the inhibition of inflammation, angiogenesis, epithelial-mesenchymal transition (EMT), cancer cell proliferation, and metastasis. In silico studies also demonstrated strong interactions between chamuangone and BCLM-related targets, along with encouraging pharmacokinetic and toxicokinetic profiles.Conclusion: This investigation highlights the potential uses of chamuangone as a chemopreventive agent and functional component for managing the advanced stages of BC. © 2025 Society of Chemical Industry.","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Science of Food and Agriculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/jsfa.70175","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Breast cancer (BC) is a life-threatening disease, with approximately 60% of cases progressing to breast cancer lung metastasis (BCLM). Chamuangone, a key bioactive constituent derived from the leaves of the Thai plant Garcinia cowa Roxb., has demonstrated significant potential as an anticancer agent. To evaluate its anticancer effects, chamuangone was purified from the leaves and assessed through in silico, in vitro, and in vivo studies using a mouse model of BC.

Results: The results revealed that chamuangone (10 mg L^-1) inhibited cancer cell proliferation by inducing G0/G1 phase arrest and apoptosis in 4T1-luciferase (4T1-luc) and Michigan Cancer Foundation-7 (MCF-7) cells. It also modulated the levels of key apoptotic markers, including B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and caspase-3. Similarly, in vivo studies demonstrated that chamuangone significantly (P < 0.05) inhibited breast tumor growth and lung metastasis at a dose of 100 mg kg⁻¹ body weight, in comparison with the standard drug doxorubicin (5 mg kg⁻¹ body weight) in mice. Immunohistochemical analysis further revealed that chamuangone modulated several BCLM-associated markers, including estrogen receptor (ER), progesterone receptor (PR), Ki-67 antigen (Ki-67), cluster of differentiation 44 (CD44), E-cadherin, N-cadherin, vascular endothelial growth factor A (VEGFA), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), in both breast tumors and lung tissues. These effects contributed to the inhibition of inflammation, angiogenesis, epithelial-mesenchymal transition (EMT), cancer cell proliferation, and metastasis. In silico studies also demonstrated strong interactions between chamuangone and BCLM-related targets, along with encouraging pharmacokinetic and toxicokinetic profiles.

Conclusion: This investigation highlights the potential uses of chamuangone as a chemopreventive agent and functional component for managing the advanced stages of BC. © 2025 Society of Chemical Industry.

查看原文本刊更多论文

从藤黄中纯化的Chamuangone抑制乳腺癌的炎症、血管生成、上皮-间质转化和转移。

背景：乳腺癌（BC）是一种危及生命的疾病，大约60%的病例进展为乳腺癌肺转移（BCLM）。Chamuangone是从泰国植物Garcinia cowa Roxb的叶子中提取的一种关键的生物活性成分。已显示出作为抗癌剂的巨大潜力。为了评估其抗癌作用，我们从茶叶中纯化了chamuangone，并利用小鼠BC模型进行了计算机、体外和体内研究。结果：chamuangone （10 mg L-1）通过诱导4T1-luciferase （4T1-luc）和Michigan cancer Foundation-7 （MCF-7）细胞G0/G1期阻滞和凋亡抑制癌细胞增殖。它还调节关键凋亡标志物的水平，包括b细胞淋巴瘤2 （Bcl-2）、Bcl-2相关X蛋白（Bax）和caspase-3。同样，体内研究表明，chamuangone显著(P结论：本研究强调了chamuangone作为化学预防剂和功能成分管理晚期BC的潜在用途。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.