Quantification of acrylamide in infant formula via a modified QuEChERS protocol coupled with high-performance liquid chromatography-tandem mass spectrometry

IF 8.2 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry: X Pub Date : 2025-09-13 DOI:10.1016/j.fochx.2025.103026

Jiahui Ma , Xiaomin Li , Shuangxia Luo , Xianjiang Li , Qinghe Zhang , Xiong Yin

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引用次数: 0

Abstract

The accurate quantification of acrylamide within complex infant formulas remain a significant challenge. This study validates a novel method for analyzing acrylamide in infant formula, which combines the quick, easy, cheap, effective, rugged, safe (QuEChERS) pretreatment approach with the isotope dilution HPLC-MS/MS technique. The optimized QuEChERS procedure effectively eliminates co-extracted matrix interferences and achieves high extraction efficiency. Notably, this approach simultaneously accomplishes a recovery rate of 103 % to 105 %, a relative standard deviation of less than 1.6 %, and a limit of quantification at 0.90 μg/kg. The high accuracy of this proposed method is further confirmed using commercially certified reference material (CRM, No. 108–02-006). Importantly, when 20 real samples were analyzed, five were found to be contaminated with acrylamide. These results demonstrate that the proposed method is rapid and reliable for determining acrylamide levels in infant formula, suggesting its potential as a primary detection method for acrylamide in complex matrices.

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采用改进的QuEChERS方案结合高效液相色谱-串联质谱法定量婴儿配方奶粉中的丙烯酰胺

复杂婴儿配方奶粉中丙烯酰胺的准确定量仍然是一个重大挑战。本研究验证了一种将快速、简便、廉价、有效、坚固、安全（QuEChERS）预处理方法与同位素稀释HPLC-MS/MS技术相结合的分析婴儿配方奶粉中丙烯酰胺的新方法。优化后的QuEChERS程序有效地消除了共提取矩阵的干扰，实现了较高的提取效率。值得注意的是，该方法回收率为103% ~ 105%，相对标准偏差小于1.6%，定量限为0.90 μg/kg。使用商业认证的标准物质（CRM, No. 108-02-006）进一步证实了该方法的高精度。重要的是，当分析20个真实样本时，发现其中5个被丙烯酰胺污染。结果表明，该方法快速、可靠地测定了婴儿配方奶粉中丙烯酰胺的含量，有可能成为复杂基质中丙烯酰胺的主要检测方法。

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来源期刊

Food Chemistry: X CHEMISTRY, APPLIED-

CiteScore

4.90

自引率

6.60%

发文量

315

审稿时长

55 days

期刊介绍： Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.