Preparation, Characterization, and Gastric Mucosal Cell Migration Promotion of EGCG Nanoparticles Released from a Low pH Environment

IF 2.8 Q2 FOOD SCIENCE & TECHNOLOGY

ACS food science & technology Pub Date : 2025-08-20 DOI:10.1021/acsfoodscitech.5c00507

Die Jiang, Zi-Hang Ding, Jing Cheng, Xiao-Ying Yang, Yun-He Zhang, Jian Wang, Huan-Huan Xu*, Jun Sheng* and Qiang-Qiang Zhu*,

{"title":"Preparation, Characterization, and Gastric Mucosal Cell Migration Promotion of EGCG Nanoparticles Released from a Low pH Environment","authors":"Die Jiang, Zi-Hang Ding, Jing Cheng, Xiao-Ying Yang, Yun-He Zhang, Jian Wang, Huan-Huan Xu*, Jun Sheng* and Qiang-Qiang Zhu*, ","doi":"10.1021/acsfoodscitech.5c00507","DOIUrl":null,"url":null,"abstract":"(−)-Epigallocatechin-3-gallate (EGCG), as the primary bioactive constituent of tea, exhibits potential health benefits and therapeutic effects on the gastrointestinal system. However, its application is limited by its instability within the gastrointestinal tract, as well as its low systemic delivery efficiency and poor oral bioavailability. This study developed EGCG nanoparticles using bovine serum albumin (BSA) and dextran. Optimal parameters (VEGCG/VBSA/VDextran = 1:2:2) produced nanoparticles with high encapsulation efficiency (94.31%), uniform size (597.04 ± 12.6 nm), stable zeta potential (−24.63 ± 0.85 mV), and favorable morphological characteristics. These nanoparticles exhibited sustained-release properties in vitro and significant antioxidant capacity under simulated gastrointestinal conditions. Notably, they enhanced the migration of gastric epithelial cells under acidic conditions (pH 4.5). The developed nanoformulation strategy effectively addresses critical challenges in EGCG delivery by improving controlled release kinetics and promoting gastric mucosal repair mechanisms in low pH environments.","PeriodicalId":72048,"journal":{"name":"ACS food science & technology","volume":"5 9","pages":"3468–3478"},"PeriodicalIF":2.8000,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS food science & technology","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsfoodscitech.5c00507","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

(−)-Epigallocatechin-3-gallate (EGCG), as the primary bioactive constituent of tea, exhibits potential health benefits and therapeutic effects on the gastrointestinal system. However, its application is limited by its instability within the gastrointestinal tract, as well as its low systemic delivery efficiency and poor oral bioavailability. This study developed EGCG nanoparticles using bovine serum albumin (BSA) and dextran. Optimal parameters (V_EGCG/V_BSA/V_Dextran = 1:2:2) produced nanoparticles with high encapsulation efficiency (94.31%), uniform size (597.04 ± 12.6 nm), stable zeta potential (−24.63 ± 0.85 mV), and favorable morphological characteristics. These nanoparticles exhibited sustained-release properties in vitro and significant antioxidant capacity under simulated gastrointestinal conditions. Notably, they enhanced the migration of gastric epithelial cells under acidic conditions (pH 4.5). The developed nanoformulation strategy effectively addresses critical challenges in EGCG delivery by improving controlled release kinetics and promoting gastric mucosal repair mechanisms in low pH environments.

Abstract Image

查看原文本刊更多论文

低pH环境释放的EGCG纳米颗粒的制备、表征和胃粘膜细胞迁移促进

（−）-表没食子儿茶素-3-没食子酸酯（EGCG），作为茶叶的主要生物活性成分，对胃肠道系统具有潜在的健康益处和治疗作用。然而，其在胃肠道内的不稳定性、较低的全身给药效率和较差的口服生物利用度限制了其应用。本研究利用牛血清白蛋白（BSA）和右旋糖酐制备EGCG纳米颗粒。最佳参数（VEGCG/VBSA/VDextran = 1:2:2）制备的纳米颗粒包封效率高（94.31%），尺寸均匀（597.04±12.6 nm）， zeta电位稳定（−24.63±0.85 mV），形态特征良好。这些纳米颗粒在体外具有缓释特性，并在模拟胃肠道条件下具有显著的抗氧化能力。值得注意的是，它们在酸性条件下（pH 4.5）增强了胃上皮细胞的迁移。开发的纳米配方策略通过改善控制释放动力学和促进低pH环境下胃粘膜修复机制，有效解决了EGCG递送的关键挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS food science & technology

CiteScore

3.30

自引率

0.00%

发文量