Gene expression signatures mediate phosphorus source effects on egg production and bone strength in late-lay hens.

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-18 DOI:10.1002/jsfa.70184

Astrid Lissette Barreto Sánchez, Minyahel Tilahun, Ivonne Salazar Bell, Uchechukwu Edna Obianwuna, Habtamu Ayalew, Sumei Cao, Haijun Zhang

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引用次数: 0

Abstract

Background: This study explores how varying dietary phosphorus levels, particularly through dicalcium phosphate (DCP) and tricalcium phosphate (TCP), influence egg production performance and bone quality in aging laying hens, along with tibia bone gene expression. A total of 576 laying hens were randomly assigned to a 2 × 4 factorial design (two phosphorus sources × four levels), with six replicates per treatment (12 hens each). Hens were housed under controlled environmental conditions. The study employed two-way analysis of variance to analyze 18 parameters related to production, egg quality, serum biochemistry, and bone traits, with post hoc Tukey tests for multiple comparisons. Egg and bone parameters were analyzed separately. Pearson's correlation assessed relationships while controlling for phosphorus level and hen age.

Results: Hens fed TCP had higher egg production and improved feed conversion ratio compared to DCP. Serum inorganic phosphorus was higher in DCP-fed hens. TCP also enhanced bone quality, with greater bone breaking strength, bone mineral density, and bone mineral content (BMC). Gene expression analysis revealed upregulated osteogenic markers (OPG and RANKL; P < 0.05) in TCP, while DCP elevated FGF23 expression (6.88). RANKL showed significant correlation with production traits: 43% with egg production (P < 0.001) and 32.1% with feed conversion (P < 0.01). These findings highlight that TCP supports bone health and calcium regulation, whereas DCP promotes bone resorption, ultimately reducing production longevity.

Conclusion: TCP boosts productivity and bone health in aging hens by upregulating OPG/RANKL signaling, optimizing bone remodeling. Future studies should investigate long-term gene expression changes and alternative pathways. © 2025 Society of Chemical Industry.

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基因表达特征介导磷源对后期蛋鸡产蛋量和骨强度的影响。

背景：本研究探讨了不同饲粮磷水平，特别是磷酸二钙（DCP）和磷酸三钙（TCP）水平对老龄蛋鸡产蛋性能和骨质量以及胫骨基因表达的影响。试验选用576只蛋鸡，随机采用2 × 4因子设计（2个磷源× 4个水平），每个处理6个重复（每个处理12只鸡）。母鸡被饲养在受控的环境条件下。本研究采用双向方差分析对产量、蛋品质、血清生化和骨骼性状等18个参数进行分析，并采用事后Tukey检验进行多重比较。卵和骨参数分别分析。在控制磷水平和母鸡年龄的情况下，Pearson相关性评估了两者之间的关系。结果：与DCP相比，TCP可提高母鸡的产蛋率和饲料系数。dcp饲粮母鸡血清无机磷含量较高。TCP还提高了骨质量，具有更大的骨折强度、骨矿物质密度和骨矿物质含量（BMC）。结论：TCP通过上调OPG/RANKL信号，促进衰老母鸡的生产能力和骨骼健康，优化骨重塑。未来的研究应探讨长期的基因表达变化和替代途径。©2025化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.