Absence of Glaucoma in Tg-MYOCY437H Mice of Diverse Genetic Backgrounds.

IF 4.7 2区医学 Q1 OPHTHALMOLOGY

Investigative ophthalmology & visual science Pub Date : 2025-09-02 DOI:10.1167/iovs.66.12.40

Nicholas G Tolman, Marina Simón, Felicia A Juarez, Chi Zhang, Zhivka I Hristova, Tionna B Ouellette, Michael A Sellarole, Wilhelmine N deVries, Christa Montgomery, Simon W M John

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Abstract

Purpose: Mutations in the myocilin (MYOC) gene cause elevated intraocular pressure and glaucoma. To better understand the factors influencing susceptibility to glaucoma, we studied the MYOC mutation (MYOCY437H).

Methods: We characterized ocular phenotypes in Tg-MYOCY437H mice on nine different mouse strain backgrounds.

Results: No glaucoma-related phenotypes were observed. We detected neither elevated intraocular pressure (daytime readings) nor optic nerve degeneration differences between wild type (WT) and Tg-MYOCY437H mice. This included an absence of Tg-MYOCY437H-induced phenotypes on a mixed B6SJL background that best reflected the original publications with this strain. We confirmed that this result was not due to an absence of transgene expression in ocular tissues.

Conclusions: Our data indicate undefined complexity and that the previously reported glaucoma phenotypes are not robust across all institutions and environments.

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不同遗传背景的Tg-MYOCY437H小鼠青光眼不存在

目的：心肌蛋白（MYOC）基因突变可引起眼压升高和青光眼。为了更好地了解影响青光眼易感性的因素，我们研究了MYOC突变（MYOCY437H）。方法：在9种不同的小鼠品系背景下，对Tg-MYOCY437H小鼠的眼部表型进行了表征。结果：未观察到青光眼相关表型。我们在野生型（WT）和Tg-MYOCY437H小鼠之间既没有检测到眼压升高（白天读数），也没有检测到视神经变性的差异。这包括在混合B6SJL背景下缺乏tg - myocy437h诱导的表型，这最好地反映了该菌株的原始出版物。我们证实这一结果不是由于在眼组织中缺乏转基因表达。结论：我们的数据显示了未定义的复杂性，并且先前报道的青光眼表型在所有机构和环境中并不稳定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Investigative ophthalmology & visual science 医学-眼科学

CiteScore

6.90

自引率

4.50%

发文量

339

审稿时长

1 months

期刊介绍： Investigative Ophthalmology & Visual Science (IOVS), published as ready online, is a peer-reviewed academic journal of the Association for Research in Vision and Ophthalmology (ARVO). IOVS features original research, mostly pertaining to clinical and laboratory ophthalmology and vision research in general.