{"title":"Prevalence and effective treatment of drug-resistant Pseudomonas aeruginosa biofilm using flavonoid naringin.","authors":"Subramanian Palaniappan, Vinothini Gunasekaran, Thavasumani Muthu, Lekha Sree Venkatesan, Palanivel Sathishkumar","doi":"10.1007/s00449-025-03232-3","DOIUrl":null,"url":null,"abstract":"<p><p>Pseudomonas aeruginosa infections and biofilms are difficult to treat due to their remarkable ability of antibiotic resistance. Consequently, the incidence of drug-resistant P. aeruginosa infection cases is steadily increasing in hospitals worldwide. Herein, the frequency of drug-resistant P. aeruginosa wound infection cases among hospitalized patients is examined. In addition, suitable natural components with potent antibacterial activity against the drug-resistant P. aeruginosa strains are screened. Among 27 specimens, three P. aeruginosa strains (including carbapenem and multidrug-resistant) are isolated. The antibacterial efficacy of natural components, such as curcumin, naringin, quercetin, tannic acid, and rutin, is evaluated against the isolated drug-resistant P. aeruginosa strains. Comparatively, naringin showed great antibacterial potential against drug-resistant P. aeruginosa strains. The zone of inhibition is found between 13 and 16 mm for naringin (20 mM) against P. aeruginosa strains; while, the minimum inhibitory concentration is found between 10 and 14 mM. A complete eradication of bacterial cells in P. aeruginosa mature biofilm is achieved by naringin at 28 mM within 24 h. Naringin interacts with the most important amino acids found in the MexR and RlpA, which confirms its role in the targeted treatment of drug-resistant P. aeruginosa. Remarkably, naringin is found to be hemocompatible up to 30 mM. Overall, this study suggests that naringin might be an outstanding biocompatible natural component to effectively treat multidrug-resistant P. aeruginosa infections and biofilms.</p>","PeriodicalId":9024,"journal":{"name":"Bioprocess and Biosystems Engineering","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprocess and Biosystems Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s00449-025-03232-3","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Pseudomonas aeruginosa infections and biofilms are difficult to treat due to their remarkable ability of antibiotic resistance. Consequently, the incidence of drug-resistant P. aeruginosa infection cases is steadily increasing in hospitals worldwide. Herein, the frequency of drug-resistant P. aeruginosa wound infection cases among hospitalized patients is examined. In addition, suitable natural components with potent antibacterial activity against the drug-resistant P. aeruginosa strains are screened. Among 27 specimens, three P. aeruginosa strains (including carbapenem and multidrug-resistant) are isolated. The antibacterial efficacy of natural components, such as curcumin, naringin, quercetin, tannic acid, and rutin, is evaluated against the isolated drug-resistant P. aeruginosa strains. Comparatively, naringin showed great antibacterial potential against drug-resistant P. aeruginosa strains. The zone of inhibition is found between 13 and 16 mm for naringin (20 mM) against P. aeruginosa strains; while, the minimum inhibitory concentration is found between 10 and 14 mM. A complete eradication of bacterial cells in P. aeruginosa mature biofilm is achieved by naringin at 28 mM within 24 h. Naringin interacts with the most important amino acids found in the MexR and RlpA, which confirms its role in the targeted treatment of drug-resistant P. aeruginosa. Remarkably, naringin is found to be hemocompatible up to 30 mM. Overall, this study suggests that naringin might be an outstanding biocompatible natural component to effectively treat multidrug-resistant P. aeruginosa infections and biofilms.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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