Tharani T. Dissanayake, Shona Crawford-Clark, Benjamin J. Bowen, Luke C. Henderson, Colin J. Barrow, Jacqui L. Adcock
{"title":"Comparison of Methods for the Preparation of Fatty Acid Methyl Esters for the Analysis of Omega-3 Rich Oils by Gas Chromatography","authors":"Tharani T. Dissanayake, Shona Crawford-Clark, Benjamin J. Bowen, Luke C. Henderson, Colin J. Barrow, Jacqui L. Adcock","doi":"10.1002/aocs.12974","DOIUrl":null,"url":null,"abstract":"<p>Fatty acid analysis is required in a wide range of industries, and conversion to fatty acid methyl esters followed by gas chromatography is by far the most common method of analysis. However, despite widespread use, there is no consensus on which derivatization method should be used or how the methods compare. In this work, five methods for the preparation of fatty acid methyl esters were compared for the analysis of three omega-3 rich oils (marine, krill, and flaxseed oils). Overall, we found that all methods gave similar results for percentage composition of fatty acids, but some variation existed when comparing absolute quantitation of fatty acids. We also found that the derivatization efficacy of each method varied depending on lipid class, with catalyst type having a significant effect. Two methods employing: (i) methanolic hydrogen chloride and (ii) methanolic sodium hydroxide followed by a boron trihalide reagent (BCl<sub>3</sub> or BF<sub>3</sub>) had derivatizing efficacies (by lipid class) that were significantly higher than for the other methods.</p>","PeriodicalId":17182,"journal":{"name":"Journal of the American Oil Chemists Society","volume":"102 9","pages":"1351-1361"},"PeriodicalIF":2.4000,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aocs.onlinelibrary.wiley.com/doi/epdf/10.1002/aocs.12974","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Oil Chemists Society","FirstCategoryId":"97","ListUrlMain":"https://aocs.onlinelibrary.wiley.com/doi/10.1002/aocs.12974","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
Fatty acid analysis is required in a wide range of industries, and conversion to fatty acid methyl esters followed by gas chromatography is by far the most common method of analysis. However, despite widespread use, there is no consensus on which derivatization method should be used or how the methods compare. In this work, five methods for the preparation of fatty acid methyl esters were compared for the analysis of three omega-3 rich oils (marine, krill, and flaxseed oils). Overall, we found that all methods gave similar results for percentage composition of fatty acids, but some variation existed when comparing absolute quantitation of fatty acids. We also found that the derivatization efficacy of each method varied depending on lipid class, with catalyst type having a significant effect. Two methods employing: (i) methanolic hydrogen chloride and (ii) methanolic sodium hydroxide followed by a boron trihalide reagent (BCl3 or BF3) had derivatizing efficacies (by lipid class) that were significantly higher than for the other methods.
期刊介绍：
The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate.
JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
