{"title":"Modulation of Lipase Selectivity Through Conformational Engineering and Reaction Media: A Review","authors":"Jiawei Zheng, Xin Wang, Lihong He, Nanjing Zhong","doi":"10.1002/aocs.70004","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Lipase selectivity is a key factor in determining the efficiency, specificity, and applicability of lipases in various industrial processes. It is also a significant aspect in the theoretical understanding of enzymatic reactions and the design of improved biocatalysts through protein engineering. This paper presents a comprehensive review of strategies employed in conformational engineering and the selection of reaction media to modulate the selectivities of lipases. It examines various factors that influence conformational engineering, such as organic solvents, pH levels, ionic liquids, reaction temperatures, additives, and immobilization techniques. Additionally, the review analyzes the impact of reaction media, including the use of organic solvents, supercritical fluids, and ionic liquids and the means of energy transfer (microwave irradiation) on selectivity. Special emphasis is placed on the modulation of product selectivity in glycerolysis reactions by using ionic liquids as the reaction media. This review aims to provide insights for the design of enzymatic selective catalysis mediated by lipases and to lay the groundwork for understanding the mechanisms of selective catalysis at the molecular structural level. By exploring these strategies and their effects on lipase selectivity, the paper contributes to the advancement of more efficient and targeted enzymatic processes in various industrial applications.</p>\n </div>","PeriodicalId":17182,"journal":{"name":"Journal of the American Oil Chemists Society","volume":"102 9","pages":"1453-1475"},"PeriodicalIF":2.4000,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Oil Chemists Society","FirstCategoryId":"97","ListUrlMain":"https://aocs.onlinelibrary.wiley.com/doi/10.1002/aocs.70004","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
Lipase selectivity is a key factor in determining the efficiency, specificity, and applicability of lipases in various industrial processes. It is also a significant aspect in the theoretical understanding of enzymatic reactions and the design of improved biocatalysts through protein engineering. This paper presents a comprehensive review of strategies employed in conformational engineering and the selection of reaction media to modulate the selectivities of lipases. It examines various factors that influence conformational engineering, such as organic solvents, pH levels, ionic liquids, reaction temperatures, additives, and immobilization techniques. Additionally, the review analyzes the impact of reaction media, including the use of organic solvents, supercritical fluids, and ionic liquids and the means of energy transfer (microwave irradiation) on selectivity. Special emphasis is placed on the modulation of product selectivity in glycerolysis reactions by using ionic liquids as the reaction media. This review aims to provide insights for the design of enzymatic selective catalysis mediated by lipases and to lay the groundwork for understanding the mechanisms of selective catalysis at the molecular structural level. By exploring these strategies and their effects on lipase selectivity, the paper contributes to the advancement of more efficient and targeted enzymatic processes in various industrial applications.
期刊介绍：
The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate.
JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.
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